Sample (30 μg of whole cell lysate)
A:NIH-3T3
5% SDS PAGE
GTX109811 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Zebrafish tissue extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with AUTS2 antibody [N3C1], Internal (GTX109811) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AUTS2 antibody [N3C1], Internal (GTX109811) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.