Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Aconitase 2 antibody [C1C3] (GTX109736) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (20 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX109736 diluted at 1:20000

Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] (GTX109736) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 μg of Aconitase 2 antibody [C1C3] (GTX109736).
Western blot analysis was performed using Aconitase 2 antibody [C1C3] (GTX109736).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
7.5% SDS PAGE
GTX109736 diluted at 1:10000

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] (GTX109736) diluted at 1:10000.

The data was published in the journal J Biomed Sci in 2013. PMID: 24359494