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Aconitase 2 antibody [C1C3]

Orthogonal
Anti-Aconitase 2 antibody [C1C3] used in Western Blot (WB). GTX109736
Anti-Aconitase 2 antibody [C1C3] used in Western Blot (WB). GTX109736
Anti-Aconitase 2 antibody [C1C3] used in Immunoprecipitation (IP). GTX109736
Anti-Aconitase 2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109736
Anti-Aconitase 2 antibody [C1C3] used in Western Blot (WB). GTX109736
Anti-Aconitase 2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX109736
Anti-Aconitase 2 antibody [C1C3] used in Western Blot (WB). GTX109736

Cat. No. GTX109736

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Aconitase 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , rat brain

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aconitase 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949567

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aconitase 2 , ACONM , HEL-S-284 , ICRD , OCA8 , OPA9

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Aconitase 2 antibody [C1C3] used in Western Blot (WB). GTX109736

GTX109736 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] (GTX109736) diluted at 1:10000.

Anti-Aconitase 2 antibody [C1C3] used in Western Blot (WB). GTX109736

GTX109736 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
7.5% SDS PAGE
GTX109736 diluted at 1:10000

Anti-Aconitase 2 antibody [C1C3] used in Immunoprecipitation (IP). GTX109736

GTX109736 IP Image

Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 μg of Aconitase 2 antibody [C1C3] (GTX109736).
Western blot analysis was performed using Aconitase 2 antibody [C1C3] (GTX109736).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Aconitase 2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109736

GTX109736 ICC/IF Image

Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] (GTX109736) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Aconitase 2 antibody [C1C3] used in Western Blot (WB). GTX109736

GTX109736 WB Image

Sample (20 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX109736 diluted at 1:20000

Anti-Aconitase 2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX109736

GTX109736 IHC-P Image

Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Aconitase 2 antibody [C1C3] (GTX109736) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Aconitase 2 antibody [C1C3] used in Western Blot (WB). GTX109736

GTX109736 WB Image

Orthogonal

The data was published in the journal J Biomed Sci in 2013. PMID: 24359494

REFERENCE

Application Reference

Chang Jiang et al. Redox Biol 2022; 54 : 102358 A CRISPR screen identifies redox vulnerabilities for KEAP1/NRF2 mutant non-small cell lung cancer.
Application : WB
Reactivity : Human
Victoriane Peugnet et al. Antioxidants (Basel) 2022; 11 (4) Mitochondrial-Targeted Therapies Require Mitophagy to Prevent Oxidative Stress Induced by SOD2 Inactivation in Hypertrophied Cardiomyocytes.
Application : WB
Reactivity : Rat
Ward NP et al. J Exp Med 2020; 217 (6) Nicotinamide nucleotide transhydrogenase regulates mitochondrial metabolism in NSCLC through maintenance of Fe-S protein function.
Application : WB
Reactivity : Human
Nathan P. Ward et al. bioRxiv 2019; (Epub) NNT Regulates Mitochondrial Metabolism in NSCLC Through Maintenance of Fe-S Protein Function.
Application : WB
Reactivity : Human
Chen JY et al. J Biomed Sci 2013; 20 : 95 quercetin-induced cardioprotection against * cytotoxicity.
Application : WB
Reactivity : Rat
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169