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Adenylate kinase 4 antibody

Anti-Adenylate kinase 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30038
Anti-Adenylate kinase 4 antibody used in Western Blot (WB). GTX30038
Anti-Adenylate kinase 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX30038
Anti-Adenylate kinase 4 antibody used in Immunoprecipitation (IP). GTX30038

Cat. No. GTX30038

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Adenylate kinase 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human AK4 (NP_037542.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887588

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenylate kinase 4 , AK 4 , AK3 , AK3L1 , AK3L2

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Adenylate kinase 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30038

GTX30038 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30038 Adenylate kinase 4 antibody.
Blue : DAPI

Anti-Adenylate kinase 4 antibody used in Western Blot (WB). GTX30038

GTX30038 WB Image

WB analysis of various sample lysates using GTX30038 Adenylate kinase 4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Adenylate kinase 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX30038

GTX30038 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX30038 Adenylate kinase 4 antibody.
Dilution : 1:200

Anti-Adenylate kinase 4 antibody used in Immunoprecipitation (IP). GTX30038

GTX30038 IP Image

IP analysis of HepG2 cell lysate using GTX30038 Adenylate kinase 4 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

Application Reference

Jan YH et al. bioRxiv 2017; (Epub) Adenylate kinase-4 modulates oxidative stress and stabilizes HIF-1α to drive lung cancer metastasis.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399