GeneTex
United States (US)

Aldolase A antibody (HRP)

Aldolase-antibody-HRP_WB_GTX40552-1_18121409_761.jpg
Aldolase-antibody-HRP-GTX40552-WB-1_18121409_534.jpg

Cat No. GTX40552

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IP, ELISA
Reactivity Rabbit
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10mg/ml BSA, IgG and Protease free, 0.01% (w/v) Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rabbit

Immunogen

Full length protein (Rabbit muscle aldolase).

Purification

IgG fraction

Conjugation

Horseradish peroxidase(HRP)

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cytoplasmic

Background

This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.
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Database

Research Area

DATA IMAGES
Aldolase-antibody-HRP_WB_GTX40552-1_18121409_761.jpg

GTX40552 WB Image

Anti aldolase antibody (GTX40552) – Immunoprecipitation and Western Blot. 300 µl aliquots of whole anti-aldolase antiserum (GTX48869) were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are: 1. Purified aldolase 2. 300 µl antiserum with no antigen (negative control) 3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml) 4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum and an equal volume of aldolase antigen in PBS was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH.  90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added.  The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel.  Gel was stained, destained and imaged using standard protocols.  Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase.  Additional higher and lower molecular weight bands correspond to serum proteins.

Aldolase-antibody-HRP-GTX40552-WB-1_18121409_534.jpg

GTX40552 WB Image

IgG purified antibody to rabbit muscle aldolase (GTX40552) was used at a 1:1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.