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Alpha fetoprotein / AFP antibody [2A9]

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Flow cytometry (FACS). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Flow cytometry (FACS). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in IHC (Paraffin sections) (IHC-P). GTX84954
Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954

Cat No. GTX84954

Host

Mouse

Clonality

Monoclonal

Clone Name

2A9

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Dog, Monkey
Package
100 μl ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10727804

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

alpha fetoprotein , AFPD , FETA , HPAFP

Cellular Localization

Secreted

Background

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Database

Research Area

DATA IMAGES

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84954

GTX84954 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Alpha fetoprotein / AFP plasmid using GTX84954 Alpha fetoprotein / AFP antibody [2A9].

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954

GTX84954 WB Image

WB analysis of HEK293T cells transfected with Alpha fetoprotein / AFP plasmid (Right) or empty vector (Left) for 48 hrs using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Loading : 5 ug per lane

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Flow cytometry (FACS). GTX84954

GTX84954 FACS Image

FACS analysis of HEK293T cells transfected with either Alpha fetoprotein / AFP plasmid(Red) or empty vector control plasmid(Blue) using GTX84954 Alpha fetoprotein / AFP antibody [2A9].

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954

GTX84954 WB Image

WB analysis of various cell lines using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Loading : 35 ug per lane

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Flow cytometry (FACS). GTX84954

GTX84954 FACS Image

FACS analysis of Jurkat cells using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Red : Primary antibody
Blue : Negative control antibody

Anti-Alpha fetoprotein / AFP antibody [2A9] used in IHC (Paraffin sections) (IHC-P). GTX84954

GTX84954 IHC-P Image

IHC-P analysis of human liver tissue using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Alpha fetoprotein / AFP antibody [2A9] used in Western Blot (WB). GTX84954

GTX84954 WB Image

WB analysis of 3T3 cell lysate using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Loading : 10 ug per lane
Dilution : 1:200

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 389