Alpha fetoprotein / AFP antibody [2A9]

Cat. No. GTX84954

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	2A9
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Dog, Monkey
Package	100 μl ($489)

See all Alpha fetoprotein / AFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
ICC/IF	1:100
IHC-P	1:150
FACS	1:100

Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10727804

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha fetoprotein , AFPD , FETA , HPAFP

Cellular Localization

Secreted

Background

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Database

Gene ID: 174 AFP
UniProt: P02771 AFP

Research Area

DATA IMAGES

GTX84954 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Alpha fetoprotein / AFP plasmid using GTX84954 Alpha fetoprotein / AFP antibody [2A9].

GTX84954 WB Image

WB analysis of HEK293T cells transfected with Alpha fetoprotein / AFP plasmid (Right) or empty vector (Left) for 48 hrs using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Loading : 5 ug per lane

GTX84954 FACS Image

FACS analysis of HEK293T cells transfected with either Alpha fetoprotein / AFP plasmid(Red) or empty vector control plasmid(Blue) using GTX84954 Alpha fetoprotein / AFP antibody [2A9].

GTX84954 WB Image

WB analysis of 3T3 cell lysate using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Loading : 10 ug per lane
Dilution : 1:200

GTX84954 WB Image

WB analysis of various cell lines using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Loading : 35 ug per lane

GTX84954 FACS Image

FACS analysis of Jurkat cells using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Red : Primary antibody
Blue : Negative control antibody

GTX84954 IHC-P Image

IHC-P analysis of human liver tissue using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

Application Reference

Chen YH et al. Sci Rep 2019; 9 (1) : 9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.

Application : ICC/IF

Reactivity : Human

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REVIEW

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Application Reference

Chen YH et al. Sci Rep 2019; 9 (1):9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.

Application : ICC/IF

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 489

Qty

Alpha fetoprotein / AFP antibody [2A9]

Cat. No. GTX84954

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84954 ICC/IF Image

GTX84954 WB Image

GTX84954 FACS Image

GTX84954 WB Image

GTX84954 WB Image

GTX84954 FACS Image

GTX84954 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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