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Annexin V-PE Apoptosis Detection Kit

Annexin V-PE Apoptosis Detection Kit. GTX85586
Annexin V-PE Apoptosis Detection Kit. GTX85586

Cat No. GTX85586

Application ICC/IF
Package
100 assay ($439)
PRODUCT

Product Description

The Annexin V-PE Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.



‧ Detection method- Flow cytometry (Ex = 488 nm; Em = 578 nm) and fluorescence microscopy

‧ Sample type- Live cells

‧ Species reactivity- Mammalian

‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.



Features and Benefits

‧ Simple one-step procedure; takes only 10 minutes

‧ Fast and convenient, no need for fixation of cells

‧ The Annexin V-PE Apoptosis Detection Kit contains the bright orange-red PE fluorescent probe that can be easily detected by flow cytometry or fluorescence microscopy.



Kit Contents:

Annexin V-PE

1X Binding Buffer

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Storage

Store at +4ºC. Product has an expected shelf life of 12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
Annexin V-PE Apoptosis Detection Kit. GTX85586

GTX85586 ICC/IF Image

Detection of Apoptosis with Annexin V-EGFP. Apoptosis was induced in Jurkat cells by camptothecin (2 mM) for various times as indicated. Cells were collected and incubated with Annexin V-EGFP for 5 minutes according to the kit instructions. Results were analyzed by fluorescence microscopy (top panel) and flow cytometry (bottom panel).

Annexin V-PE Apoptosis Detection Kit. GTX85586

GTX85586 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

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Package List Price ($)
$ 439