Anterior Gradient 2 antibody [GT5812]

Cat. No. GTX634163
References 1
Cat. No.  GTX634163
Anti-Anterior Gradient 2 antibody [GT5812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634163
GTX634163 ICC/IF Image

Anterior Gradient 2 antibody [GT5812] detects Anterior Gradient 2 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:500.
Blue: Hoechst 33342 staining.

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Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163
GTX634163 IHC-P Image

Anterior Gradient 2 antibody [GT5812] detects secreted Anterior Gradient 2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-Anterior Gradient 2 antibody [GT5812] used in Western Blot (WB). GTX634163
GTX634163 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163
GTX634163 IHC-P Image

Anterior Gradient 2 antibody [GT5812] detects secreted Anterior Gradient 2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-Anterior Gradient 2 antibody [GT5812] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634163
Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163
Anti-Anterior Gradient 2 antibody [GT5812] used in Western Blot (WB). GTX634163
Anti-Anterior Gradient 2 antibody [GT5812] used in IHC (Paraffin sections) (IHC-P). GTX634163
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    GT5812
  • Isotype
    IgG2b
  • Applications
    WB ICC/IF IHC-P
  • Reactivity
    Human
Summary
Anterior gradient 2 (AGR2), also known as hAG-2 and Gob-4, is a member of the disulfide isomerase family of endoplasmic reticulum proteins with a predicted molecular weight of 20 kDa. AGR2 is overexpressed in human mucinous ovarian tumor tissues and in patient sera with early stage mucinous ovarian tumors. It was found to enhance tumor growth and metastasis, and is a potential tumor marker for detection of circulating tumor cells.
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