Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Anterior Gradient 2 antibody (GTX135344) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.