WB analysis of various samples using GTX23707 Ap2A antibody prior incubated with (Lane 6-10) or without (Lane 1-5) blocking peptide.
Lane 1 and 6 : HeLa nuclear extracts
Lane 2 and 7 : HeLa whole cell lysates
Lane 3 and 8 : 293T whole cell lysates
Lane 4 and 9 : A431 whole cell lysates
Lane 5 and 10 : Jurkat whole cell lysates
Loading : 20 μg
Dilution : 1:500

Western blot using GeneTex's Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa correspond-ing to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 μg/ml of the immunizing peptide which effect-ively blocks the specific reactivity of this antibody with AP2A. Approximately 20 μg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.