Apolipoprotein B antibody [HL1962]

Cat. No. GTX637799

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1962
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Apolipoprotein B products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

516 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Apolipoprotein B

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

apolipoprotein B , FLDB , LDLCQ4 , apoB-100 , apoB-48

Cellular Localization

Cytoplasm,Secreted

Background

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Database

Gene ID: 338 APOB
UniProt: P04114 APOB

Research Area

DATA IMAGES

GTX637799 ICC/IF Image

Apolipoprotein B antibody [HL1962] detects Apolipoprotein B protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein B stained by Apolipoprotein B antibody [HL1962] (GTX637799) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

GTX637799 ELISA Image

Indirect ELISA analysis was performed by coating the plate with purified native human ApoB protein (4000-62.5 ng/mL). Coated protein was probed with Apolipoprotein B antibody [HL1962] (GTX637799) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX637799 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Apolipoprotein B antibody [HL1962] (GTX637799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637799 IHC-P Image

Apolipoprotein B antibody [HL1962] detects Apolipoprotein B protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Apolipoprotein B stained by Apolipoprotein B antibody [HL1962] (GTX637799) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations