APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Apolipoprotein B
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
apolipoprotein B , FLDB , LDLCQ4 , apoB-100 , apoB-48
Cellular Localization
Cytoplasm,Secreted
Background
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX637799 ICC/IF Image
Apolipoprotein B antibody [HL1962] detects Apolipoprotein B protein by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Apolipoprotein B stained by Apolipoprotein B antibody [HL1962] (GTX637799) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
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GTX637799 ELISA Image
Indirect ELISA analysis was performed by coating the plate with purified native human ApoB protein (4000-62.5 ng/mL). Coated protein was probed with Apolipoprotein B antibody [HL1962] (GTX637799) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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GTX637799 WB Image
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Apolipoprotein B antibody [HL1962] (GTX637799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637799 IHC-P Image
Apolipoprotein B antibody [HL1962] detects Apolipoprotein B protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon.Apolipoprotein B stained by Apolipoprotein B antibody [HL1962] (GTX637799) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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