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Ataxin 1 antibody [2F5]

Anti-Ataxin 1 antibody [2F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80399
Anti-Ataxin 1 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX80399
Anti-Ataxin 1 antibody [2F5] used in Western Blot (WB). GTX80399
Anti-Ataxin 1 antibody [2F5] used in Flow cytometry (FACS). GTX80399

Cat No. GTX80399

Host Mouse
Clonality Monoclonal
Clone Name 2F5
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity Human
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ATXN1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ataxin 1 , ATX1 , D6S504E , SCA1

Cellular Localization

Cytoplasm,Nucleus

Background

The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This loc

Database

Research Area

DATA IMAGES
Anti-Ataxin 1 antibody [2F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80399

GTX80399 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX80399 Ataxin 1 antibody [2F5].
Green : Ataxin 1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Ataxin 1 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX80399

GTX80399 IHC-P Image

IHC-P analysis of ovarian cancer tissue (left) and lung cancer tissue (right) using GTX80399 Ataxin 1 antibody [2F5].

Anti-Ataxin 1 antibody [2F5] used in Western Blot (WB). GTX80399

GTX80399 WB Image

WB analysis of HEK293 (1) and ATXN1(AA: 645-815)-hIgGFc transfected HEK293 (2) cell lysate using GTX80399 Ataxin 1 antibody [2F5].

Anti-Ataxin 1 antibody [2F5] used in Flow cytometry (FACS). GTX80399

GTX80399 FACS Image

FACS analysis of Jurkat cells using GTX80399 Ataxin 1 antibody [2F5].
Green : Ataxin 1
Purple : negative control

REFERENCE
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REVIEW
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Package List Price ($)
$ 319