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Aurora A antibody

Anti-Aurora A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134467

Cat. No. GTX134467

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Aurora A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aurora A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887291

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aurora kinase A , AIK , ARK1 , AURA , BTAK , PPP1R47 , STK15 , STK6 , STK7

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Aurora A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134467

GTX134467 ICC/IF Image

Aurora A antibody detects Aurora A protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Aurora A stained by Aurora A antibody (GTX134467) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169