Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5 - 2 μg/mL |
0.5 - 5 μg/mL |
0.5 - 5 μg/mL |
0.5 - 2 μg/mL |
Not tested in other applications.
Calculated MW
Product Note
This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
Form
Liquid
Buffer
PBS, 1% BSA, 50% Glycerol
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A peptide corresponding to BRAF V600E mutant
Purification
Protein A purified
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_2887722
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
BRAF,BRAF1,BRaf protooncogene, serine/threonine kinase,Braf,NS7,RAFB1,B-Raf,B-Raf proto-oncogene, serine/threonine kinase
Cellular Localization
Nucleus,Cytoplasm,Cell membrane
Background
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
Database
Research Area