GeneTex
United States (US)

B-Raf (V600E Mutant Specific) antibody [RM8]

GTX33595_20160517_IHC-P_18121410_902.jpg
GTX33595_20160517_IHC-P_1_18121410_559.jpg
GTX33595_20160517_IHC-P_2_18121410_977.jpg
GTX33595_20160517_WB_18121410_543.jpg
GTX33595_20160517_ELISA_18121410_221.jpg

Cat No. GTX33595

Host Rabbit
Clonality Monoclonal
Clone Name RM8
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA, IHC
Reactivity Human
APPLICATION

Application Note

Immunohistochemistry (IHC): 2 – 10 ug/mL, Immunocytochemistry (ICC): 2 – 10 ug/mL, Western Blot (WB): 0.5 – 2 ug/mL

Calculated MW

84 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A peptide corresponding to BRAF V600E mutant

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B-Raf Proto-Oncogene, Serine/Threonine Kinase,B-Raf1,B-Raf,Braf1,Ns7,Rafb1,Braf

Background

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX33595_20160517_IHC-P_18121410_902.jpg

GTX33595 IHC-P Image

Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections.

GTX33595_20160517_IHC-P_1_18121410_559.jpg

GTX33595 IHC-P Image

Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. WiDr Cells (400X, Positive)

GTX33595_20160517_IHC-P_2_18121410_977.jpg

GTX33595 IHC-P Image

Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. SW40 Cells (400X, Negative)

GTX33595_20160517_WB_18121410_543.jpg

GTX33595 WB Image

Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein.

GTX33595_20160517_ELISA_18121410_221.jpg

GTX33595 ELISA Image

Sandwich ELISA performed on the lysates of 293T cells overexpressing BRAF V600E or BRAF wild type His-tag proteins, shows the specificity of RM8 to the V600E mutation. His-tag fusion proteins were captured by an Anti-His-Tag antibody.

REFERENCE
Package List Price ($)
$ 519