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B-Raf (V600E Mutant Specific) antibody [RM8]

Orthogonal
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in Western Blot (WB). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

Cat. No. GTX33595

Reference 2

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM8

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($649)
See all B-Raf products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ICC/IF 0.5 - 5 μg/mL
IHC-P 0.5 - 5 μg/mL
ELISA 0.5 - 2 μg/mL
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Product Note

This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to BRAF V600E mutant

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2887722

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BRAF,BRAF1,BRaf protooncogene, serine/threonine kinase,Braf,NS7,RAFB1,B-Raf,B-Raf proto-oncogene, serine/threonine kinase

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of formalin fixed and paraffin embedded sections of WiDr cells (B-Raf V600E mutant) using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of formalin fixed and paraffin embedded sections of SW40 cells (B-Raf Wide type) using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of thyroid Carcinoma tissue using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in Western Blot (WB). GTX33595

GTX33595 WB Image

Orthogonal

WB analysis of cells expressing endogenous mutant (V600E) BRAF or wild type (WT) protein using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of melanoma tissue using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of thyroid Carcinoma tissue using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

REFERENCE

Application Reference

Makboul R et al. Nucl Med Commun 2020; 41 (5) : 416-425 Do BRAFV600E mutation and sodium-iodide symporter expression affect the response to radioactive iodine therapy in patients with papillary thyroid carcinoma?
Application : IHC-P
Reactivity : Human
Hansen CH et al. Proc Natl Acad Sci U S A 2017; (Epub) Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection.
Application : ELISA
Submit a Reference

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Package List Price ($)
$ 649