Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody [N1C3] (GTX109159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody [N1C3] (GTX109159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Sample (30 ug of whole cell lysate)

A: NIH-3T3

12% SDS PAGE

BCL10 antibody

GTX109159 diluted at 1:1000

BCL10 antibody [N1C3] immunoprecipitates BCL10 protein in IP experiments.
IP samples: Raji whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody [N1C3] (GTX109159)
12 % SDS-PAGE
The immunoprecipitated BCL10 protein was detected by BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Cross-linked ChIP was performed with MCF-7 chromatin extract treated with TNF-a (10 ng/ml for 30 min) and 5 μg of either control rabbit IgG or anti-BCL10 antibody. The precipitated DNA was detected by PCR with primer set targeting to IκBα.