APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human BCL10 recombinant protein (epitope aa122-168)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL10 , Bcell CLL/lymphoma 10 , CARMEN , CIPER , CLAP , IMD37 , cE10 , mE10 , BCL10 immune signaling adaptor
Cellular Localization
Cytoplasm,Perinuclear region,Membrane raft
Background
This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Database
Research Area
DATA IMAGES
![Anti-BCL10 antibody [SPM520] used in IHC (Paraffin sections) (IHC-P). GTX34424](/templates/default/js/lightbox/images/loading.gif "Anti-BCL10 antibody [SPM520] used in IHC (Paraffin sections) (IHC-P). GTX34424")
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GTX34424 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX34424 BCL10 antibody [SPM520].
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REFERENCE
There are currently no references for BCL10 antibody [SPM520] (GTX34424). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BCL10 antibody [SPM520] (GTX34424). Be the first to share your experience with this product.
![Anti-BCL10 antibody [SPM520] used in IHC (Paraffin sections) (IHC-P). GTX34424](/upload/website/prouct_img/small/GTX34424/GTX34424_20200115_IHC-P_1261_w_23060801_120.webp "Anti-BCL10 antibody [SPM520] used in IHC (Paraffin sections) (IHC-P). GTX34424")
![Anti-BCL10 antibody [SPM520] used in IHC (Paraffin sections) (IHC-P). GTX34424](/upload/website/prouct_img/normal/GTX34424/GTX34424_20200115_IHC-P_1261_w_23060801_120.webp "Anti-BCL10 antibody [SPM520] used in IHC (Paraffin sections) (IHC-P). GTX34424")