*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Jurkat , K562 , THP-1 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12
Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human BCL6. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
BCL6 transcription repressor,BCL5,BCL6A,LAZ3,ZBTB27,ZNF51
The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]
GTX101338 IHC-Fr Image
BCL6 antibody [N2C1], Internal detects BCL6 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: BCL6 stained by BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
GTX101338 WB Image
BCL6 antibody [N2C1], Internal detects BCL6 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
BCL6 antibody [N2C1], Internal (GTX101338) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101338 IP Image
BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of BCL6 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody (GTX101338) 7.5% SDS-PAGE The immunoprecipitated BCL6 protein was detected by BCL6 antibody (GTX101338) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
GTX101338 ChIP assay Image
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.
ChIP experiment and primer designs are based on Blood. 2007 Sep 15;110(6):2067-74.
GTX101338 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human tonsil, using BCL6(GTX101338) antibody(10 μg/ml).
GTX101338 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX101338 diluted at 1:1000