BCL6 antibody [N2C1], Internal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.

BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of BCL6 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody (GTX101338) 7.5% SDS-PAGE The immunoprecipitated BCL6 protein was detected by BCL6 antibody (GTX101338) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BCL6 antibody (GTX101338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.