GeneTex
United States (US)

BCL6 antibody [N2C1], Internal

GTX101338_41024_20180125_IHC-Fr_M_19011506_169.jpg
GTX101338_40163_WB_M_19011506_581.jpg
GTX101338_40163_IP_19011506_482.jpg
GTX101338_40163_ChIP_19011506_402.jpg
GTX101338_40163_IHC_19011506_184.jpg
GTX101338_41024_WB_19011506_247.jpg

Cat No. GTX101338

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr, IP, ChIP assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

Jurkat , K562 , THP-1 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCL6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B Cell Cll/Lymphoma 6,Bcl5,Bcl6A,Laz3,Zbtb27,Znf51,Bcl6

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101338_41024_20180125_IHC-Fr_M_19011506_169.jpg

GTX101338 IHC-Fr Image

BCL6 antibody [N2C1], Internal detects BCL6 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: BCL6 stained by BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101338_40163_WB_M_19011506_581.jpg

GTX101338 WB Image

BCL6 antibody [N2C1], Internal detects BCL6 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
BCL6 antibody [N2C1], Internal (GTX101338) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101338_40163_IP_19011506_482.jpg

GTX101338 IP Image

BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of BCL6 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody (GTX101338) 7.5% SDS-PAGE The immunoprecipitated BCL6 protein was detected by BCL6 antibody (GTX101338) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX101338_40163_ChIP_19011506_402.jpg

GTX101338 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.
ChIP experiment and primer designs are based on Blood. 2007 Sep 15;110(6):2067-74.

GTX101338_40163_IHC_19011506_184.jpg

GTX101338 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human tonsil, using BCL6(GTX101338) antibody(10 μg/ml).

GTX101338_41024_WB_19011506_247.jpg

GTX101338 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: K562
C: THP-1
7.5% SDS PAGE
GTX101338 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf