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BCL6 antibody [N2C1], Internal

Anti-BCL6 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in Western Blot (WB). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in Western Blot (WB). GTX101338

Cat No. GTX101338

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr, IP, ChIP assay
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

Jurkat , K562 , THP-1 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCL6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BCL6 transcription repressor,BCL5,BCL6A,LAZ3,ZBTB27,ZNF51

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-BCL6 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX101338

GTX101338 IHC-Fr Image

BCL6 antibody [N2C1], Internal detects BCL6 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: BCL6 stained by BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-BCL6 antibody [N2C1], Internal used in Western Blot (WB). GTX101338

GTX101338 WB Image

BCL6 antibody [N2C1], Internal detects BCL6 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
BCL6 antibody [N2C1], Internal (GTX101338) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCL6 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX101338

GTX101338 IP Image

BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of BCL6 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody (GTX101338) 7.5% SDS-PAGE The immunoprecipitated BCL6 protein was detected by BCL6 antibody (GTX101338) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-BCL6 antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX101338

GTX101338 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.
ChIP experiment and primer designs are based on Blood. 2007 Sep 15;110(6):2067-74.

Anti-BCL6 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX101338

GTX101338 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human tonsil, using BCL6(GTX101338) antibody(10 μg/ml).

Anti-BCL6 antibody [N2C1], Internal used in Western Blot (WB). GTX101338

GTX101338 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: K562
C: THP-1
7.5% SDS PAGE
GTX101338 diluted at 1:1000

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169