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BDNF antibody

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in Western Blot (WB). GTX132621
Anti-BDNF antibody used in Western Blot (WB). GTX132621
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in Western Blot (WB). GTX132621
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132621
Anti-BDNF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132621

Cat No. GTX132621

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

mouse brain , rat brain , BDNF-transfected 293T

Predict Reactivity

Bovine, Cat, Dog, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BDNF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

brain derived neurotrophic factor,ANON2,BULN2

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: BDNF protein stained by BDNF antibody (GTX132621) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-BDNF antibody used in Western Blot (WB). GTX132621

GTX132621 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX132621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in Western Blot (WB). GTX132621

GTX132621 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX132621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat hippocampus.
BDNF stained by BDNF antibody (GTX132621) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in Western Blot (WB). GTX132621

GTX132621 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX132621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
BDNF antibody (GTX132621) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat hippocampus.
BDNF stained by BDNF antibody (GTX132621) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132621

GTX132621 ICC/IF Image

BDNF antibody detects BDNF protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BDNF stained by BDNF antibody (GTX132621) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-BDNF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132621

GTX132621 ICC/IF Image

BDNF antibody detects BDNF protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BDNF stained by BDNF antibody (GTX132621) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169