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BDNF antibody

Orthogonal Overexpression
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132621
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in Western Blot (WB). GTX132621
Anti-BDNF antibody used in Western Blot (WB). GTX132621
Anti-BDNF antibody used in Western Blot (WB). GTX132621
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

Cat. No. GTX132621

Reference 19
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BDNF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

BDNF-transfected 293T

Predict Reactivity

Zebrafish, Bovine, Cat, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BDNF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886695

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

brain derived neurotrophic factor , ANON2 , BULN2

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: BDNF protein stained by BDNF antibody (GTX132621) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
BDNF stained by BDNF antibody (GTX132621) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
BDNF antibody (GTX132621) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132621

GTX132621 ICC/IF Image

BDNF antibody detects BDNF protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BDNF stained by BDNF antibody (GTX132621) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat hippocampus.
BDNF stained by BDNF antibody (GTX132621) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
BDNF stained by BDNF antibody (GTX132621) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in Western Blot (WB). GTX132621

GTX132621 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX132621) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in Western Blot (WB). GTX132621

GTX132621 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX132621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in Western Blot (WB). GTX132621

GTX132621 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX132621) diluted at 1:125. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
BDNF stained by BDNF antibody (GTX132621) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
BDNF stained by BDNF antibody (GTX132621) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX132621

GTX132621 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat hippocampus.
BDNF stained by BDNF antibody (GTX132621) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Umeda T et al. Geroscience 2024; 46 (2) : 1971-1987 Hawaiian native herb Mamaki prevents dementia by ameliorating neuropathology and repairing neurons in four different mouse models of neurodegenerative diseases.
XL Wang et al. Research Square 2024; Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide
NB Tran et al. PLoS One 2023; 18 (9) : e0291191 Effects of Gryllus bimaculatus and Oxya chinensis sinuosa extracts on brain damage via blood-brain barrier control and apoptosis in mice with pentylenetetrazol-induced epilepsy
Application : WB
Reactivity : Mouse
Kaushik M et al. eNeuro 2023; 10 (4) Environment Enrichment Facilitates Long-Term Memory Consolidation through Behavioral Tagging.
Application : WB
Reactivity : Rat
Lee YZ et al. Int J Mol Sci 2023; 24 (7) Neuroprotective Effects of Lactobacillus plantarum PS128 in a Mouse Model of Parkinson's Disease: The Role of Gut Microbiota and MicroRNAs.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

BDNF antibody Cat. No. GTX132621

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-BDNF antibody used in Western Blot (WB). GTX132621
Date : Anonymous submitted on 12-Jul-2023
Score :
Application Tested : WB
Sample Species : Rat
Sample : CTX TNA2 cells with/without treatment
Amount used : 20μg
Blocking : 3% BSA in TBST, 24°C, 1-2Hr
Primary Antibody : 1:1000, 4°C, >10Hr
Anti-BDNF antibody used in Western Blot (WB). GTX132621
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat cerebral cortex tissue lysates
Blocking : 5% skim milk in TBS 0.1% Tween-20, 25°C, 0.5Hr
Primary Antibody : 1:1000, 18°C, 4Hr
Submit a Review
Application Reference
Umeda T et al. Geroscience 2024; 46 (2):1971-1987 Hawaiian native herb Mamaki prevents dementia by ameliorating neuropathology and repairing neurons in four different mouse models of neurodegenerative diseases.
XL Wang et al. Research Square 2024; Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide
NB Tran et al. PLoS One 2023; 18 (9):e0291191 Effects of Gryllus bimaculatus and Oxya chinensis sinuosa extracts on brain damage via blood-brain barrier control and apoptosis in mice with pentylenetetrazol-induced epilepsy
Application : WB
Reactivity : Mouse
Kaushik M et al. eNeuro 2023; 10 (4) Environment Enrichment Facilitates Long-Term Memory Consolidation through Behavioral Tagging.
Application : WB
Reactivity : Rat
Lee YZ et al. Int J Mol Sci 2023; 24 (7) Neuroprotective Effects of Lactobacillus plantarum PS128 in a Mouse Model of Parkinson's Disease: The Role of Gut Microbiota and MicroRNAs.
Application : WB
Reactivity : Mouse
Kim HI et al. Neurotherapeutics 2022; 19 (4):1298-1312 ERRγ Ligand Regulates Adult Neurogenesis and Depression-like Behavior in a LRRK2-G2019S-associated Young Female Mouse Model of Parkinson's Disease.
Lim J et al. Front Pharmacol 2022; 13:1062650 Differentiated HT22 cells as a novel model for in vitro screening of serotonin reuptake inhibitors.
Application : WB, ICC/IF
Reactivity : Mouse
Obata H et al. Exp Ther Med 2022; 24 (5):682 Age?related differences for expression of the nerve?specific proteins after peripheral nerve injury.
Application : IHC-P
Reactivity : Mouse
Takei Y et al. Geroscience 2022; 44 (4):2305-2318 Alteration in peritoneal cells with the chemokine CX3CL1 reverses age-associated impairment of recognition memory.
Application : IHC-Fr
Reactivity : Mouse
Tsai CW et al. Neurotherapeutics 2022; 19 (2):649-659 Transcranial Ultrasound Stimulation Reverses Behavior Changes and the Expression of Calcium-Binding Protein in a Rodent Model of Schizophrenia.
Fang Yan et al. BMC Cardiovasc Disord 2022; 22 (1):182 H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure.
Application : WB
Reactivity : Rat
Guang-Ang Tian et al. Oncoimmunology 2021; 10 (1):2003956 BDNF expression in GISTs predicts poor prognosis when associated with PD-L1 positive tumor-infiltrating lymphocytes.
Application : IHC-P
Reactivity : Human
Lee LC et al. Mol Brain 2021; 14 (1):152 Association of CaMK2A and MeCP2 signaling pathways with cognitive ability in adolescents.
Application : WB
Reactivity : Human
Otsuka T et al. Stem Cells Dev 2021; Comparisons of Neurotrophic Effects of Mesenchymal Stem Cells Derived from Different Tissues on Chronic Spinal Cord Injury Rats.
Hsu TW et al. Biomaterials 2021; 272:120765 Transplantation of 3D MSC/HUVEC spheroids with neuroprotective and proangiogenic potentials ameliorates ischemic stroke brain injury.
Hung TH et al. Brain Pathol 2021;:e12968 Antenatal low-intensity pulsed ultrasound reduces neurobehavioral deficits and brain injury following dexamethasone-induced intrauterine growth restriction.
Application : WB
Reactivity : Rat
CH Chang et al. Mol Ther Nucleic Acids 2020; 20:812-822 MicroRNA-206 Regulates Stress-Provoked Aggressive Behaviors in Post-weaning Social Isolation Mice
Application : IHC-Fr
Chang JW et al. Cereb Cortex 2020; 30 (8):4597-4606 Ultrasound Stimulation Suppresses LPS-Induced Proinflammatory Responses by Regulating NF-κB and CREB Activation in Microglial Cells.
Application : WB
Reactivity : Rat
Cao K et al. Invest Ophthalmol Vis Sci 2020; 61 (3):13 Protection of the Retinal Ganglion Cells: Intravitreal Injection of Resveratrol in Mouse Model of Ocular Hypertension.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169