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BIN1 antibody

Comparison
Anti-BIN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103259
Anti-BIN1 antibody used in Western Blot (WB). GTX103259
Anti-BIN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103259
Anti-BIN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103259
Anti-BIN1 antibody used in Western Blot (WB). GTX103259
Anti-BIN1 antibody used in Western Blot (WB). GTX103259

Cat. No. GTX103259

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all BIN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BIN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165763

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

bridging integrator 1 , AMPH2 , AMPHL , CNM2 , SH3P9

Cellular Localization

Isoform BIN1: Nucleus , Isoform IIA: Cytoplasm

Background

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BIN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103259

GTX103259 ICC/IF Image

BIN1 antibody detects BIN1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BIN1 stained by BIN1 antibody (GTX103259) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-BIN1 antibody used in Western Blot (WB). GTX103259

GTX103259 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody (GTX103259) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BIN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103259

GTX103259 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using BIN1(GTX103259) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BIN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103259

GTX103259 IHC-P Image

BIN1 antibody detects BIN1 protein at cytoplasm in human papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human papillary adenocarcinoma .
BIN1 antibody (GTX103259) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BIN1 antibody used in Western Blot (WB). GTX103259

GTX103259 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with BIN1 antibody (GTX103259) diluted at 1:1000 and competitor's antibody (#13679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-BIN1 antibody used in Western Blot (WB). GTX103259

GTX103259 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody (GTX103259) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Antonio P A Ferreira et al. Nat Commun 2021; 12 (1) : 2424 Cdk5 and GSK3β inhibit fast endophilin-mediated endocytosis.
Application : WB, ICC/IF
Reactivity : Human
Chan Wah Hak L et al. Nat Cell Biol 2018; (Epub) FBP17 and CIP4 recruit SHIP2 and lamellipodin to prime the plasma membrane for fast endophilin-mediated endocytosis.
Application : IHC
Reactivity : Human
Borgo C et al. Sci Rep 2017; 7 : 42409 Generation and quantitative proteomics analysis of CK2α/α'(-/-) cells.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169