GeneTex
United States (US)

BIN1 antibody

GTX103259_40730_WB_M_Kidney_19011506_539.jpg
GTX103259_40730_WB_19011506_776.jpg
GTX103259_40730_IHC_19011506_524.jpg
GTX103259_40730_20151223_IHC-P_19011506_672.jpg

Cat No. GTX103259

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

A431 , mouse kidney

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BIN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AMPH2 antibody, AMPHL antibody, DKFZp547F068 antibody, MGC10367 antibody, SH3P9 antibody, BIN1 antibody, box-dependent myc-interacting protein 1 antibody, amphiphysin-like protein antibody, amphiphysin II antibody, box dependant MYC interacting protein 1 antibody, myc box-dependent-interacting protein 1 antibody, bridging integrator 1 antibody

Cellular Localization

Isoform BIN1: Nucleus , Isoform IIA: Cytoplasm

Background

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103259_40730_WB_M_Kidney_19011506_539.jpg

GTX103259 WB Image

Sample (50 µg of whole cell lysate)
A: mouse kidney
7.5% SDS PAGE
GTX103259 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103259_40730_WB_19011506_776.jpg

GTX103259 WB Image

Sample (30 µg of whole cell lysate)
A: A431
10% SDS PAGE
GTX103259 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103259_40730_IHC_19011506_524.jpg

GTX103259 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using BIN1(GTX103259) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103259_40730_20151223_IHC-P_19011506_672.jpg

GTX103259 IHC-P Image

BIN1 antibody detects BIN1 protein at cytoplasm in human papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human papillary adenocarcinoma .
BIN1 antibody (GTX103259) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf