GeneTex
United States (US)

BORIS antibody

GTX32476_20180530_ICCIF_18121410_870.jpg
GTX32476_20180530_WB_18121410_972.jpg

Cat No. GTX32476

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
Not tested in other applications.

Calculated MW

76 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CTCFL (NP_001255970.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ccctc-Binding Factor Like,Boris,Ct27,Ctcf-T,Hmgb1L1,Dj579F20.2,Ctcfl

Background

CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Database

Research Area

DATA IMAGES
GTX32476_20180530_ICCIF_18121410_870.jpg

GTX32476 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX32476 BORIS antibody.
Blue : DAPI

GTX32476_20180530_WB_18121410_972.jpg

GTX32476 WB Image

WB analysis of various samples using GTX32476 BORIS antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329