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BORIS antibody

Anti-BORIS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32476
Anti-BORIS antibody used in Western Blot (WB). GTX32476

Cat No. GTX32476

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CTCFL (NP_001255970.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CCCTC-binding factor like , BORIS , CT27 , CTCF-T , HMGB1L1 , dJ579F20.2

Cellular Localization

Cytoplasm,Nucleus

Background

CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Database

Research Area

DATA IMAGES

Anti-BORIS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32476

GTX32476 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX32476 BORIS antibody.
Blue : DAPI

Anti-BORIS antibody used in Western Blot (WB). GTX32476

GTX32476 WB Image

WB analysis of various samples using GTX32476 BORIS antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

There are currently no references for BORIS antibody (GTX32476). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 329