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BRCA1 antibody [17F8] - ChIP grade

Anti-BRCA1 antibody [17F8] - ChIP grade used in Immunoprecipitation (IP). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in ChIP assay (ChIP assay). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

Cat No. GTX70111

Host Mouse
Clonality Monoclonal
Clone Name 17F8
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay, IHC
Reactivity Human, Mouse
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1 μg/ml
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

220 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Specificity/Sensitivity

This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 762-1315.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Brca1, Dna Repair Associated,Brcai,Brcc1,Brovca1,Fancs,Iris,Pnca4,Ppp1R53,Pscp,Rnf53,Brca1

Cellular Localization

Nuclear/Cytoplasmic

Background

BRCA1-17F8 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the Aexon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.

Database

Research Area

DATA IMAGES
Anti-BRCA1 antibody [17F8] - ChIP grade used in Immunoprecipitation (IP). GTX70111

GTX70111 IP Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay.
6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract.
Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [17F8] - ChIP grade used in ChIP assay (ChIP assay). GTX70111

GTX70111 ChIP assay Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay.
The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay.
Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319