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BRCA1 antibody [17F8] - ChIP grade

Anti-BRCA1 antibody [17F8] - ChIP grade used in Immunoprecipitation (IP). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in ChIP assay (ChIP assay). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111
Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

Cat No. GTX70111

Host

Mouse

Clonality

Monoclonal

Clone Name

17F8

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay, IHC

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1 μg/ml
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

208 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Product Note

This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 762-1315.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368627

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-BRCA1 antibody [17F8] - ChIP grade used in Immunoprecipitation (IP). GTX70111

GTX70111 IP Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay.
6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract.
Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [17F8] - ChIP grade used in ChIP assay (ChIP assay). GTX70111

GTX70111 ChIP assay Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay.
The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay.
Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BRCA1 antibody [17F8] - ChIP grade used in Western Blot (WB). GTX70111

GTX70111 WB Image

BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

BRCA1 antibody [17F8] - ChIP grade Cat No. GTX70111

Rating ( Average 5 based on 6 users reviews)
Application
Western Blot(WB) ( Average 5 based on 5 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Breast Cancer Res in 2001.PMID: 11250747
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2016.PMID: 27764250
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2016.PMID: 27764250
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2016.PMID: 27764250
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2016.PMID: 27764250
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2016.PMID: 27764250
Application Reference
SDS
PBS.pdf