United States (US)

BRCA1 antibody [MS110]

Cat No. GTX16780

Host Mouse
Clonality Monoclonal
Clone Name MS110
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human

Application Note

For IHC-Fr, IHC-P, IP, IF: Use at an assay dependent dilution. For WB: Use at a concentration of 1.5 - 2.5 μg/ml. Predicted molecular weight: 207 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

208 kDa. ( Note )

Positive Control

MCF7 cells


GTX16780 doesnot cross-react with blood group antigens or growth factor receptors such as EGFR.




50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5 with =0.1% sodium azide


Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.


0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species



Recombinant full length protein (Human).


From hybridoma culture supernatant




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


Brca1, Dna Repair Associated,Brcai,Brcc1,Brovca1,Fancs,Iris,Pnca4,Ppp1R53,Pscp,Rnf53,Brca1

Cellular Localization



BRCA1 (breast and ovarian cancer susceptibility protein 1) is a RING finger protein containing a BRCT domain. BRCA1 exists as a heterodimer with 22 possible isoforms. The full length protein has a reported molecular weight of 208 kD. BRCA1 localizes to the mitotic spindle microtubules, centriole walls, pericentriolar fibers at centrosomes. Unphosphorylated BRCA1 localizes on chromosomes from metaphase through telophase; phosphorylated BRCA1 resides in inner chromosomal structure, centrosome, cleavage furrow during prophase through telophase, and relocalizes to the perinuclear region when cells are subjected to IR or UV radiation in S phase. BRCA1 acts as a tumor suppressor and can function as a secreted growth inhibitory protein, participate in transcription coupled repair of oxidative DNA damage, X-chromosome inactivation, and can function as a E3 ubiquitin ligase. BRCA1 can be transcriptionally downregulated by Ets-2, Brg-1, and Hmga-1. BRCA1 can be modified by glycosylation, ubiquitination and phosphorylation by CDK4, ATM/ATR, cdk2, and hChk2. The BRCA1 protein has been reported to interact with RNA polymerase II holoenzyme and BARD1. BRCA1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA1 protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA1 function confers an increased susceptibility for both breast and ovarian cancer.


Research Area