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BRF2 antibody [N1C1]

Anti-BRF2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116223
Anti-BRF2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116223
Anti-BRF2 antibody [N1C1] used in Western Blot (WB). GTX116223

Cat. No. GTX116223

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all BRF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

A549 , HeLa , HCT116

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BRF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621404

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRF2 RNA polymerase III transcription initiation factor subunit , BRFU , TFIIIB50

Background

This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BRF2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116223

GTX116223 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BRF2(GTX116223) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BRF2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116223

GTX116223 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRF2(GTX116223) antibody at 1:200 dilution.

Anti-BRF2 antibody [N1C1] used in Western Blot (WB). GTX116223

GTX116223 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: Hela
C: HCT116
10% SDS PAGE
GTX116223 diluted at 1:1000

REFERENCE

Application Reference

Li Y et al. Transl Lung Cancer Res 2021; 10 (4) : 1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169