Boiled and unboiled mouse liver extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BSEP antibody [N3C1], Internal (GTX102608) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using BSEP(GTX102608) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BSEP antibody [N3C1], Internal (GTX102608) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

BSEP antibody [N3C1], Internal detects ABCB11 protein at membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ABCB11 protein stained by BSEP antibody [N3C1], Internal (GTX102608) diluted at 1:500.
Blue: Hoechst 33343 staining.

BSEP antibody [N3C1], Internal detects BSEP protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
BSEP stained by BSEP antibody [N3C1], Internal (GTX102608) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min