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BUB1 antibody

Anti-BUB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31758
Anti-BUB1 antibody used in Western Blot (WB). GTX31758
Anti-BUB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31758

Cat. No. GTX31758

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all BUB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 - 4 μg/mL.
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

A-20 Cell Slide(GTX25541) , A20 Cell Lysate(GTX27180)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.The immunogen is located within amino acids 40 - 90 of Bub1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BUB1 mitotic checkpoint serine/threonine kinase , BUB1A , BUB1L , hBUB1

Cellular Localization

Nucleus

Background

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-BUB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31758

GTX31758 ICC/IF Image

ICC/IF analysis of A-20 cells using GTX31758 BUB1 antibody.
Working concentration : 10 μg/ml

Anti-BUB1 antibody used in Western Blot (WB). GTX31758

GTX31758 WB Image

WB analysis of A-20 cell lysate using GTX31758 BUB1 antibody.
Working concentration : (A) 2 and (B) 4 μg/ml

Anti-BUB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31758

GTX31758 ICC/IF Image

ICC/IF analysis of A-20 cells using GTX31758 BUB1 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399