APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:50 - 1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine136 (S-R-Sp-A-P).
Purification
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
RRID
AB_11171739
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BAD,BBC2,BCL2 associated agonist of cell death,BCL2L8,Bad
Cellular Localization
Mitochondrion outer membrane,Cytoplasm
Background
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX79125 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX79125 Bad (phospho Ser136) antibody.
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REFERENCE
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REVIEW
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