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Bassoon antibody [SAP7F407]

Anti-Bassoon antibody [SAP7F407] used in Immunohistochemistry (IHC). GTX13249
Anti-Bassoon antibody [SAP7F407] used in Western Blot (WB). GTX13249

Cat No. GTX13249

Host Mouse
Clonality Monoclonal
Clone Name SAP7F407
Isotype IgG2a
Application WB, ICC/IF, IP, IHC
Reactivity Mouse, Rat
Package
50 μg ($319)
APPLICATION

Application Note

ICC: Use at a concentration of 0.5 - 5 μg/ml. IHC: Use at a concentration of 2 μg/ml. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 400 kDa (predicted molecular weight: 472.6 kDa). Additional bands between 97 and 400 kDa are also identified and are reported to be proteolytic degradation products of Bassoon. Optimal dilutions/concentrations should be determined by the end user.
PROPERTIES

Form

Liquid

Buffer

Preservative: None. Constituents: 50% Glycerol, PBS, 0.1mM PMSF

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant rat bassoon fragment (residues 738-1035) expressed asa fusion protein in E.coli.

Purification

Protein G purified
This antibody has been affinity purified.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bassoon (Presynaptic Cytomatrix Protein),Znf231,Bsn

Background

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96%. The human BASSOON gene has recently been cloned and localized. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors.Another presynaptic protein, Piccolo was found to colocalize with Bassoon in cultured hippocampal neurons. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.

Research Area

DATA IMAGES
Anti-Bassoon antibody [SAP7F407] used in Immunohistochemistry (IHC). GTX13249

GTX13249 IHC Image

Immunohistochemistry analysis of bassoon localization in the rat brain cerebellar molecular layer using Bassoon, mAb (SAP7F407)

Anti-Bassoon antibody [SAP7F407] used in Western Blot (WB). GTX13249

GTX13249 WB Image

"Western blot analysis of Bassoon Lane 1: MW marker Lane 2: Rat Brain Tissue Lysate"

REFERENCE
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Package List Price ($)
$ 319