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Bcl-2 antibody [N1N2], N-term

Overexpression
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

Cat No. GTX100064

Reference 55
3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Zebrafish
See all Bcl-2 products;
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

THP-1 , HL-60 , mouse spleen , *PA-1 , *OKT3

Predict Reactivity

Cat, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2, Apoptosis Regulator,Bcl-2,Ppp1R50,Bcl2

Cellular Localization

Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-Bcl-2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100064

GTX100064 ICC/IF Image

Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bcl-2 stained by Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064

GTX100064 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2(GTX100064) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064

GTX100064 IHC-P Image

Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE

Application Reference

Yuan F et al. Cancer Lett 2019; 446 : 1月14日 TIPE3 is a regulator of cell apoptosis in glioblastoma.
Application : WB
Reactivity : Human
Batsaikhan B et al. Int J Mol Sci 2019; 20 (3) Post-Injury Neuroprotective Effects of the Thalidomide Analog 3,6'-Dithiothalidomide on Traumatic Brain Injury.
Application : IHC-P
Reactivity : Rat
Yu H et al. Microcirculation 2019; : e12537 Effect of mild hypothermia on cerebral microcirculation in a murine cardiopulmonary resuscitation model.
Application : WB
Reactivity : Rat
Abdel-Daim MM et al. Sci Total Environ 2019; 650 (Pt 2) : 2063-2073 Hepatorenal protective effects of taurine and N-acetylcysteine against fipronil-induced injuries: The antioxidant status and apoptotic markers expression in rats.
Application : IHC-P
Reactivity : Rat
Lee CW et al. J Cell Physiol 2018; (Epub ahead of print) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Application : WB
Reactivity : Human
Show More +
Submit a Reference
REVIEW

Bcl-2 antibody [N1N2], N-term Cat No. GTX100064

Rating
( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 3 users reviews)
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysateA:Untreated MG63 whole cell lysate
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Date : Anonymous submitted on 09-Jan-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : OKT3 cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Date : Anonymous submitted on 08-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : PA-1 cells
Amount used : 50μg
Blocking : 5% milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Submit a Review
Application Reference
Yuan F et al. Cancer Lett 2019; 446:1月14日 TIPE3 is a regulator of cell apoptosis in glioblastoma.
Application : WB
Reactivity : Human
Batsaikhan B et al. Int J Mol Sci 2019; 20 (3) Post-Injury Neuroprotective Effects of the Thalidomide Analog 3,6'-Dithiothalidomide on Traumatic Brain Injury.
Application : IHC-P
Reactivity : Rat
Yu H et al. Microcirculation 2019;:e12537 Effect of mild hypothermia on cerebral microcirculation in a murine cardiopulmonary resuscitation model.
Application : WB
Reactivity : Rat
Abdel-Daim MM et al. Sci Total Environ 2019; 650 (Pt 2):2063-2073 Hepatorenal protective effects of taurine and N-acetylcysteine against fipronil-induced injuries: The antioxidant status and apoptotic markers expression in rats.
Application : IHC-P
Reactivity : Rat
Lee CW et al. J Cell Physiol 2018; (Epub ahead of print) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Application : WB
Reactivity : Human
Si J et al. Toxicology 2018; (Epub ahead of print) Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.
Application : WB
Reactivity : Zebrafish
Wang TH et al. Int J Mol Sci 2018; 19 (9) Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction.
Application : WB
Reactivity : Human
Lee CF et al. Biomedicine (Taipei) 2018; 8 (3):15 Benzyl isothiocyanate (BITC) triggers mitochondria-mediated apoptotic machinery in human cisplatin-resistant oral cancer CAR cells.
Application : WB
Reactivity : Human
Jianghe Cui et al. Int J Clin Exp Med 2018; 11 (8):8275-8281 MiR-365 promotes hepatoma cell apoptosis through regulating DJ-1-PTEN/PI3K/AKT signaling pathway.
Application : WB
Reactivity : Human
Ho Y et al. Food Chem Toxicol 2018; 120:346-355 Resveratrol inhibits human leiomyoma cell proliferation via crosstalk between integrin αvβ3 and IGF-1R.
Application : WB
Reactivity : Human
Jui-Cheng Tsai et al. Journal of Functional Foods 2018; 48:283-296 Extracts of Cerbera manghas L. effectively inhibit the viability of glioblastoma cell lines and their cancer stemloids in vitro and in mouse xenograft model.
Application : WB
Reactivity : Human
Pengfei Deng et al. International Journal of Science 2018; 5:5 Hsp90 inhibitor SNX-2112 inhibits cell growth by regulating Bcl-2 family proteins and cyclin proteins in MCF-7 cells.
Application : WB
Reactivity : Human
Ran S et al. Int Endod J 2018; (Epub ahead of print) Enterococcus faecalis induces apoptosis and pyroptosis of human osteoblastic MG63 cells via the NLRP3 inflammasome.
Application : WB
Reactivity : Human
Xiao YF et al. J Cell Mol Med 2018; (Epub ahead of print) FKBP12.6 protects heart from AngII-induced hypertrophy through inhibiting Ca2+ /calmodulin-mediated signalling pathways in-vivo and in-vitro.
Application : WB
Reactivity : Mouse
Dong T et al. Biomedicine & Pharmacotherapy 2018; 102:874-883 The protective roles of L-borneolum, D-borneolum and synthetic borneol in cerebral ischaemia via modulation of the neurovascular unit.
Application : IHC-P
Reactivity : Rat
Chen PY et al. Int J Mol Sci 2018; 19 (4) Response of Myeloid Leukemia Cells to Luteolin is Modulated by Differentially Expressed Pituitary Tumor-Transforming Gene 1 (PTTG1) Oncoprotein.
Application : WB
Reactivity : Human
Kim SH et al. Biomedicine & Pharmacotherapy 2018; 99:997-1008 Teroxirone suppresses growth and motility of human hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Chang HP et al. Int J Oncol 2018; (Epub ahead of print) Pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in cisplatin-resistant human oral cancer CAR cells via AKT signaling.
Application : WB
Reactivity : Human
Chung YC et al. Sci Rep 2018; 8 (1):3930 Metformin-induced caveolin-1 expression promotes T-DM1 drug efficacy in breast cancer cells.
Application : WB
Reactivity : Human
Gunes-Bayir A et al. Mol Cell Biochem 2018; (Epub ahead of print) Effects of carvacrol on human fibroblast (WS-1) and gastric adenocarcinoma (AGS) cells in vitro and on Wistar rats in vivo.
Application : IHC-P
Reactivity : Rat
Azmy MS et al. Mol Neurobiol 2018; (Epub ahead of print) Neuroprotective Effects of Filgrastim in Rotenone-Induced Parkinson's Disease in Rats: Insights into its Anti-Inflammatory, Neurotrophic, and Antiapoptotic Effects.
Application : IHC-P
Reactivity : Rat
Lin SC et al. Oncol Rep 2018; 39 (2):703-710 Glycyrrhizic acid induces human MDA-MB-231 breast cancer cell death and autophagy via the ROS-mitochondrial pathway.
Application : WB
Yang JS et al. Oncol Rep 2018; 39 (1):383-391 ITR-284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells.
Wang JP et al. Oncol Lett 2017; 14 (3):3503-3509 Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
Li J et al. Stem Cell Res Ther 2017; 8 (1):107 Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model.
Lee SO et al. J Ginseng Res 2017; (Epub ahead of print) Korean red ginseng enhances pneumococcal Δpep27 vaccine efficacy by inhibiting reactive oxygen species production.
Application : WB
Reactivity : Mouse
Toraih EA et al. Tumour Biol 2017; 39 (11):1.01E+15 MicroRNA-target cross-talks: Key players in glioblastoma multiforme.
Application : IHC-P
Reactivity : Human
Lin GL et al. PLoS One 2017; 12 (10):e0185625 Modulation of the mRNA-binding protein HuR as a novel reversal mechanism of epirubicin-triggered multidrug resistance in colorectal cancer cells.
Application : WB
Reactivity : Human
Wu JS et al. Mol Neurobiol 2017; (Epub ahead of print) Clinacanthus nutans Mitigates Neuronal Apoptosis and Ischemic Brain Damage Through Augmenting the C/EBPβ-Driven PPAR-γ Transcription.
Application : WB
Reactivity : Mouse
Chen HY et al. Int J Mol Sci 2017; 18 (10) Isoliquiritigenin Induces Autophagy and Inhibits Ovarian Cancer Cell Growth.
Application : WB
Reactivity : Human
Tsui KH et al. Reproduction 2017; 154 (2):101-110 DHEA protects mitochondria against dual modes of apoptosis and necroptosis in human granulosa HO23 cells.
Application : WB
Reactivity : Human
Gao Z et al. Hindawi 2017; (Epub ahead of print) Dab1 contributes to angiotensin II-induced apoptosis via p38 signaling pathway in podocytes.
Application : WB
Reactivity : Mouse
Eid W et al. J Cell Biochem 2017; (Epub ahead of print) Neferine Enhances the Antitumor Effect of Mitomycin-C in Hela Cells Through the Activation of p38-MAPK Pathway.
Application : WB
Reactivity : Human
Lee CC et al. PLoS One 2017; 12 (3):e0173890 The microRNA-302b-inhibited insulin-like growth factor-binding protein 2 signaling pathway induces glioma cell apoptosis by targeting nuclear factor IA.
Application : WB
Reactivity : Human
Hsu SW et al. BMC Complement Altern Med 2017; 17 (1):29 Rhodiola crenulata extract counteracts the effect of hypobaric hypoxia in rat heart via redirection of the nitric oxide and arginase 1 pathway.
Application : WB
Reactivity : Rat
Liu Y et al. Technol Cancer Res Treat 2016; 15 (3):487-97 Effects of Oxymatrine on the Proliferation and Apoptosis of Human Hepatoma Carcinoma Cells.
Application : IHC-P
Reactivity : Mouse
Chen MF et al. BMC Cancer 2016; 16:532 Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
Application : WB
Reactivity : Human
Chen PH et al. Neuropharmacology 2016; 110 (Pt A):362-75 The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Application : WB
Reactivity : Human
Chen HH et al. Sci Rep 2016; 6:27945 Urinary miR-16 transactivated by C/EBPβ reduces kidney function after ischemia/reperfusion-induced injury.
Application : WB
Reactivity : Human
Y Zhang et al. Journal of Materials Chemistry B 2016; (Epub ahead of print) Surface modification with E-cadherin fusion protein for mesenchymal stem cell culture.
Application : WB
Reactivity : Human
Buchkremer S et al. JND 2016; 3 (2):169-181 Sil1-Mutant Mice Elucidate Chaperone Function in Neurological Disorders.
Application : IHC-P
Reactivity : Mouse
Fu X et al. PLoS One 2016; 11 (2):e0148819 High-Dose Fluoride Impairs the Properties of Human Embryonic Stem Cells via JNK Signaling.
Application : WB
Reactivity : Human
Huang CC et al. Oncotarget 2015; 6 (11):8698-708 Paxillin promotes colorectal tumor invasion and poor patient outcomes via ERK-mediated stabilization of Bcl-2 protein by phosphorylation at Serine 87.
Application : WB, IHC-P
Reactivity : Human
Lu JW et al. Med Oncol 2015; 32 (7):206 MK-2206 induces apoptosis of AML cells and enhances the cytotoxicity of cytarabine.
Application : WB
Reactivity : Human
Li CJ et al. Sci Rep 2015; (Epub ahead of print) Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Application : WB
Reactivity : Human
Hsu WH et al. PLoS One 2015; 10 (4):e0121298 Evaluation of the medicinal herb Graptopetalum paraguayense as a treatment for liver cancer.
Application : WB
Reactivity : Human
Szu-Ting Lin et al. Cellular and Molecular Life Sciences 2015; 72 (12):2395-409 PGRMC1 contributes to doxorubicin-induced chemoresistance in MES-SA uterine sarcoma.
Application : WB
Reactivity : Human
J. Abdi et al. The Journal of Nutritional Biochemistry 2014; Omega-3 fatty acids, EPA and DHA induce apoptosis and enhance drug sensitivity in multiple myeloma cells but not in normal peripheral mononuclear cells
Application : WB
Reactivity : Human
Pu YF et al. Int J Clin Exp Pathol 2014; 7 (4):1563-71 Generation of homologous cell pairs using the oral lymphatic system.
Application : WB
Reactivity : Human
Lee JG et al. Toxicol In Vitro 2014; 28 (4):562-570 Acrylamide-induced apoptosis in rat primary astrocytes and human astrocytoma cell lines.
Application : WB
Reactivity : Human, Rat
Abdi J et al. Blood Cancer J 2013; 3:e119 Stimulation of Toll-like receptor-1/2 combined with Velcade increases cytotoxicity to human multiple myeloma cells.
Application : WB
Reactivity : Human
Wu DW et al. Oncogene 2013; Phosphorylation of paxillin confers cisplatin resistance in non-small cell lung cancer via activating ERK-mediated Bcl-2 expression.
Application : WB, IHC-P
Reactivity : Human
Chen YW et al. Evid Based Complement Alternat Med 2013; 2013:364519 Cardioprotective Effects of Quercetin in Cardiomyocyte under Ischemia/Reperfusion Injury.
Application : WB
Reactivity : Rat
Li CJ et al. Evid Based Complement Alternat Med 2012; 2012:261971 Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways.
Application : WB
Reactivity : Human
Liu CH et al. J Ethnopharmacol 2011; 137 (1):503-11 Subamolide A, a component isolated from Cinnamomum subavenium, induces apoptosis mediated by mitochondria-dependent, p53 and ERK1/2 pathways in human urothelial carcinoma cell line NTUB1.
Application : WB
Reactivity : Human
SDS
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Glycerol.pdf
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