PRODUCT
Summary
BCL-2 antibody recognizes BCL-2 protein. BCL-2 (26 kDa) regulates mitochondrial dynamics, is involved in the regulation of mitochondrial fusion and fission, and is associated with apoptosis. Abnormal expression of BCL-2 protein is linked to various cancers, autoimmunity, and psychiatric disorders. This BCL-2 antibody is a widely published rabbit polyclonal validated for multiple applications.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
THP-1 , HL-60 , mouse spleen , *PA-1 , *OKT3
Predict Reactivity
Cat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036326
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BCL2 apoptosis regulator , Bcl-2 , PPP1R50
Cellular Localization
Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100064 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100064 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100064 ICC/IF Image
Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Bcl-2 stained by Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX100064 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX100064 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100064 WB Image
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100064 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX100064 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2(GTX100064) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100064 IHC-P Image
Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
Bcl-2 antibody [N1N2], N-term
Cat No. GTX100064
Rating
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( Average 4.9 based on 18 users reviews)
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Application
Western Blot(WB)
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( Average 4.9 based on 16 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2020.
PMID: 32089770
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Front Pharmacol in 2017.
PMID: 29326587
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2013.
PMID: 23573126
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Stem Cell Res Ther in 2017.
PMID: 28482861
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Biomolecules in 2020.
PMID: 32635653
Date :
Anonymous submitted on 05-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
U2OS cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 12Hr
Figure Note :
0-30 μM cisplatin treatment for 24hr.
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2019.
PMID: 31210840
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal J Cell Mol Med in 2018.
PMID: 29682889
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25849560
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2016.
PMID: 26859149
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2012.
PMID: 23091557
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Apoptosis in 2020.
PMID: 32100210
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Apoptosis in 2020.
PMID: 32100210
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-P
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Apoptosis in 2020.
PMID: 32100210
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-P
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Apoptosis in 2020.
PMID: 32100210
Date :
Anonymous submitted on 26-May-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
MG63 cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Lane Description :
Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysateA:Untreated MG63 whole cell lysate
Date :
Anonymous submitted on 09-Jan-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
OKT3 cells
Amount used :
50μg
Blocking :
5% skim milk, 25°C, 1Hr
Primary Antibody :
1:1000, 25°C, 1Hr
Date :
Anonymous submitted on 08-Dec-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
PA-1 cells
Amount used :
50μg
Blocking :
5% milk, 25°C, 0.5Hr
Primary Antibody :
1:2000, 4°C, 16Hr