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Bcl-2 antibody [N1N2], N-term

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064
Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

Cat No. GTX100064

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Zebrafish
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

BCL-2 antibody recognizes BCL-2 protein. BCL-2 (26 kDa) regulates mitochondrial dynamics, is involved in the regulation of mitochondrial fusion and fission, and is associated with apoptosis. Abnormal expression of BCL-2 protein is linked to various cancers, autoimmunity, and psychiatric disorders. This BCL-2 antibody is a widely published rabbit polyclonal validated for multiple applications.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

THP-1 , HL-60 , mouse spleen , *PA-1 , *OKT3

Predict Reactivity

Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036326

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2 apoptosis regulator , Bcl-2 , PPP1R50

Cellular Localization

Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100064

GTX100064 ICC/IF Image

Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bcl-2 stained by Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064

GTX100064 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2(GTX100064) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Bcl-2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100064

GTX100064 IHC-P Image

Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Bcl-2 antibody [N1N2], N-term used in Western Blot (WB). GTX100064

GTX100064 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Bcl-2 antibody [N1N2], N-term Cat No. GTX100064

Rating ( Average 4.9 based on 18 users reviews)
Application
Western Blot(WB) ( Average 4.9 based on 16 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2017.PMID: 29326587
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2013.PMID: 23573126
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2017.PMID: 28482861
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2020.PMID: 32635653
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM cisplatin treatment for 24hr.
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2019.PMID: 31210840
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2018.PMID: 29682889
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25849560
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 26859149
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23091557
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Apoptosis in 2020.PMID: 32100210
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Apoptosis in 2020.PMID: 32100210
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Apoptosis in 2020.PMID: 32100210
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Apoptosis in 2020.PMID: 32100210
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysateA:Untreated MG63 whole cell lysate
Date : Anonymous submitted on 09-Jan-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : OKT3 cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Date : Anonymous submitted on 08-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : PA-1 cells
Amount used : 50μg
Blocking : 5% milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf