GeneTex
United States (US)

Bcl-X antibody

GTX105661_40051_IFA_19011506_773.jpg
GTX105661_40051_WB_19011506_462.jpg
GTX105661_40051_20160125_IHC-P_19011506_638.jpg
GTX105661_40051_WB_R_19011506_331.jpg
GTX105661_WB_M_19011506_510.jpg
GTX105661_IHC_19011506_939.jpg
GTX105661_40051_IP_19011506_273.jpg
GTX105661_40051_20150410_IFA_19011506_524.jpg

Cat No. GTX105661

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HCT116 , NIH-3T3 , PC-12 , *OKT3

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bcl-X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BCL-XL/S antibody, BCL2L antibody, BCLX antibody, BCLXL antibody, BCLXS antibody, Bcl-X antibody, DKFZp781P2092 antibody, bcl-xL antibody, bcl-xS antibody, BCL2L1 antibody, apoptosis regulator Bcl-X antibody, bcl-2-like protein 1 antibody, protein phosphatase 1, regulatory subunit 52 antibody, BCL2-like 1 antibody

Cellular Localization

Mitochondrion membrane; Single-pass membrane protein , Nucleus membrane; Single-pass membrane protein; Cytoplasmic side , Mitochondrion membrane , Nucleus membrane

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105661_40051_IFA_19011506_773.jpg

GTX105661 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using BCL-x(GTX105661) antibody at 1:500 dilution.

GTX105661_40051_WB_19011506_462.jpg

GTX105661 WB Image

Sample (30 µg of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX105661 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105661_40051_20160125_IHC-P_19011506_638.jpg

GTX105661 IHC-P Image

Bcl-X antibody detects Bcl-X protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Bcl-X antibody (GTX105661) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105661_40051_WB_R_19011506_331.jpg

GTX105661 WB Image

Bcl-X antibody detects BCL2L1 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
12% SDS-PAGE
Bcl-X antibody (GTX105661) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105661_WB_M_19011506_510.jpg

GTX105661 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX105661 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105661_IHC_19011506_939.jpg

GTX105661 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCL2L1(GTX105661) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105661_40051_IP_19011506_273.jpg

GTX105661 IP Image

Immunoprecipitation of Bcl-X protein from HCT-116 whole cell extracts using 5 μg of Bcl-X antibody (GTX105661).
Western blot analysis was performed using Bcl-X antibody (GTX105661).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105661_40051_20150410_IFA_19011506_524.jpg

GTX105661 ICC/IF Image

Bcl-X antibody detects Bcl-X protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bcl-X protein stained by Bcl-X antibody (GTX105661) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

Bcl-X antibody Cat No. GTX105661

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 09-Jan-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : OKT3 cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf