APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
HCT116 , NIH-3T3 , PC-12 , *OKT3
Predict Reactivity
Rabbit, Sheep, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.95 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Bcl-X. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949733
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BCL2 like 1 , BCL-XL/S , BCL2L , BCLX , Bcl-X , PPP1R52
Cellular Localization
Mitochondrion membrane; Single-pass membrane protein , Nucleus membrane; Single-pass membrane protein; Cytoplasmic side , Mitochondrion membrane , Nucleus membrane
Background
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX105661 WB Image
HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Bcl-X antibody (GTX105661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX105661 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A549, using BCL-x(GTX105661) antibody at 1:500 dilution.
|
|
GTX105661 IHC-P Image
Bcl-X antibody detects Bcl-X protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Bcl-X antibody (GTX105661) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX105661 WB Image
Bcl-X antibody detects BCL2L1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
Bcl-X antibody (GTX105661) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX105661 WB Image
Sample (30 μg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX105661 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX105661 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCL2L1(GTX105661) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX105661 IP Image
Immunoprecipitation of Bcl-X protein from HCT-116 whole cell extracts using 5 μg of Bcl-X antibody (GTX105661).
Western blot analysis was performed using Bcl-X antibody (GTX105661) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
|
|
GTX105661 ICC/IF Image
Bcl-X antibody detects Bcl-X protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bcl-X protein stained by Bcl-X antibody (GTX105661) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
|
REFERENCE
REVIEW
Bcl-X antibody
Cat No. GTX105661
|
Rating
|
|
( Average 5 based on 2 users reviews)
|
Application
|
Western Blot(WB)
|
|
( Average 5 based on 2 users reviews)
|
Date :
Anonymous submitted on 05-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
U2OS cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 12Hr
Figure Note :
0-30 μM cisplatin treatment for 24hr.
Date :
Anonymous submitted on 09-Jan-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
OKT3 cells
Amount used :
50μg
Blocking :
5% skim milk, 25°C, 1Hr
Primary Antibody :
1:1000, 25°C, 1Hr
