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Bestrophin antibody

Anti-Bestrophin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX14927
Anti-Bestrophin antibody used in Western Blot (WB). GTX14927
Anti-Bestrophin antibody used in IHC (Paraffin sections) (IHC-P). GTX14927
Anti-Bestrophin antibody used in IHC (Frozen sections) (IHC-Fr). GTX14927

Cat. No. GTX14927

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($409)
See all Bestrophin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:200
ELISA 1:10000
IHC 1:100
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine, 0.5% BSA, 30% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

N-epitope Synthetic peptide corresponding to unique amino acid sequences on human Bestrophin 1 protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_368466

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bestrophin 1 , ARB , BEST , BMD , Best1V1Delta2 , RP50 , TU15B , VMD2

Cellular Localization

Cell membrane

Background

This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]

Database

Research Area

DATA IMAGES

Anti-Bestrophin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX14927

GTX14927 ICC/IF Image

ICC/IF analysis of cells (basolateral cell membranes) using GTX14927 Bestrophin antibody.
Dilution : 1:100

Anti-Bestrophin antibody used in Western Blot (WB). GTX14927

GTX14927 WB Image

WB analysis of Bestrophin recombinant protein using GTX14927 Bestrophin antibody.
Dilution : 1:500

Anti-Bestrophin antibody used in IHC (Paraffin sections) (IHC-P). GTX14927

GTX14927 IHC-P Image

IHC-P analysis of mouse retina tissue using GTX14927 Bestrophin antibody.
Dilution : 1:50

Anti-Bestrophin antibody used in IHC (Frozen sections) (IHC-Fr). GTX14927

GTX14927 IHC-Fr Image

The data was published in the journal PLoS One in 2013.PMID: 23437266

REFERENCE

Application Reference

Tochitani S et al. PLoS One 2013; 8 (2) : e56901 Immunoreactivity for GABA, GAD65, GAD67 and Bestrophin-1 in the meninges and the choroid plexus: implications for non-neuronal sources for GABA in the developing mouse brain.
Application : IHC
Reactivity : Mouse
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Package List Price ($)
$ 409