GeneTex
United States (US)

Bestrophin antibody

Cat No. GTX14927

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC
Reactivity Human, Mouse, Rat
See all Bestrophin products
APPLICATION

Application Note

For ICC: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For IP: Use at a dilution of 1:200. For WB: Use at a dilution of 1:500. Optimal dilutions/concentrations should be determined by the researcher
PROPERTIES

Form

Liquid

Buffer

Tris-glycine buffer pH7.2-7.4, stabilizing proteins , cryogenic stabilizers, glycerol and 0.02% sodium azide as preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide - amino acids 31-52 of Human Bestrophin. The peptide was amidated for conjugation.

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BEST, VMD2, Bestrophin, 7439, O76090, 153700, TU15B, BMD

Cellular Localization

Plasma membrane

Background

Bestrophin is a 68 kDa basolateral plasma membrane protein encoded by the VMD2 gene. The role of Bestrophin is as yet unclear but it is thought to be involved in Best Macular Dystrophy (BMD) or Vitelliform Macular Dystrophy (VMD2); inherited early onset autosomal forms of macular degeneration. There is evidence to suggest that Bestrophin is a chloride channel that plays a role in generating the altered electrooculogram in Best disease patients.
REFERENCE

Application Reference

Tochitani S et al. PLoS One 2013; 8 (2) : e56901 Immunoreactivity for GABA, GAD65, GAD67 and Bestrophin-1 in the meninges and the choroid plexus: implications for non-neuronal sources for GABA in the developing mouse brain.
Application : IHC
Reactivity : Mouse
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Package List Price ($)
$ 319