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Bestrophin antibody

Anti-Bestrophin antibody used in IHC (Paraffin sections) (IHC-P). GTX14927
Anti-Bestrophin antibody used in Western Blot (WB). GTX14927
Anti-Bestrophin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX14927

Cat No. GTX14927

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC

Reactivity

Human, Mouse, Rat
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:100
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:200
ELISA 1:10000
IHC 1:100
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine pH7.4-7.9, 30% Glycerol, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

N-epitope Synthetic peptide corresponding to unique amino acid sequences on human Bestrophin 1 protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_368466

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

bestrophin 1 , ARB , BEST , BMD , Best1V1Delta2 , RP50 , TU15B , VMD2

Cellular Localization

Cell membrane

Background

This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]

Database

DATA IMAGES

Anti-Bestrophin antibody used in IHC (Paraffin sections) (IHC-P). GTX14927

GTX14927 IHC-P Image

IHC-P analysis of mouse retina tissue using GTX14927 Bestrophin antibody.
Dilution : 1:50

Anti-Bestrophin antibody used in Western Blot (WB). GTX14927

GTX14927 WB Image

WB analysis of Bestrophin recombinant protein using GTX14927 Bestrophin antibody.
Dilution : 1:500

Anti-Bestrophin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX14927

GTX14927 ICC/IF Image

ICC/IF analysis of cells (basolateral cell membranes) using GTX14927 Bestrophin antibody.
Dilution : 1:100

REFERENCE

REVIEW

Bestrophin antibody Cat No. GTX14927

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Elife in 2018. PMID: 30511641
Package List Price ($)
$ 379