APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Rat2 , Raw264.7 , C2C12
Product Note
IP/MS validation was supported by references (PMID:30377403)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human Bid Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949744
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BH3 interacting domain death agonist , FP497
Cellular Localization
Cytoplasm , Mitochondrion membrane , BH3-interacting domain death agonist p15: Mitochondrion membrane , BH3-interacting domain death agonist p13: Mitochondrion membrane , Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Isoform 2: Mitochondrion membrane
Background
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX110568 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110568 ICC/IF Image
Bid antibody [N1C3-2] detects Bid protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bid protein stained by Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.
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GTX110568 ICC/IF Image
Bid antibody [N1C3-2] detects BID protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: BID protein stained by Bid antibody [N1C3-2] (GTX110568) diluted at 1:500. Blue: Hoechst 33343 staining.
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GTX110568 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Bid(GTX110568) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110568 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000.
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GTX110568 IP Image
Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3-2] (GTX110568) or Bid antibody [N1C3] (GTX101323). Western blot analysis was performed using Bid antibody [N1C3-2] (GTX110568) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX110568 WB Image
Bid antibody [N1C3-2] detects Bid protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg H1299 whole cell lysate/extract D. 30 μg HeLa whole cell lysate/extract E. 30 μg HepG2 whole cell lysate/extract F. 30 μg Molt-4 whole cell lysate/extract G. 30 μg Raji whole cell lysate/extract 7.5 % SDS-PAGE Bid antibody [N1C3-2] (GTX110568) dilution: 1:1000
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GTX110568 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.
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REFERENCE
REVIEW
Bid antibody [N1C3-2]
Cat. No. GTX110568
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 03-Oct-2022
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the 2016 in Oncotarget.
PMID: 27564258