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Bid antibody [N1C3-2]

KO/KD IP/MS
Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568
Anti-Bid antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110568
Anti-Bid antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110568
Anti-Bid antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110568
Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568
Anti-Bid antibody [N1C3-2] used in Immunoprecipitation (IP). GTX110568
Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568
Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

Cat No. GTX110568

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Bid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377403)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Bid Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BH3 interacting domain death agonist , FP497

Cellular Localization

Cytoplasm , Mitochondrion membrane , BH3-interacting domain death agonist p15: Mitochondrion membrane , BH3-interacting domain death agonist p13: Mitochondrion membrane , Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Isoform 2: Mitochondrion membrane

Background

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

GTX110568 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bid antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110568

GTX110568 ICC/IF Image

Bid antibody [N1C3-2] detects Bid protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bid protein stained by Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Bid antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110568

GTX110568 ICC/IF Image

Bid antibody [N1C3-2] detects BID protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: BID protein stained by Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-Bid antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110568

GTX110568 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Bid(GTX110568) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

GTX110568 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000.

Anti-Bid antibody [N1C3-2] used in Immunoprecipitation (IP). GTX110568

GTX110568 IP Image

Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3-2] (GTX110568) or Bid antibody [N1C3] (GTX101323).
Western blot analysis was performed using Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

GTX110568 WB Image

Bid antibody [N1C3-2] detects Bid protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg H1299 whole cell lysate/extract
D. 30 μg HeLa whole cell lysate/extract
E. 30 μg HepG2 whole cell lysate/extract
F. 30 μg Molt-4 whole cell lysate/extract
G. 30 μg Raji whole cell lysate/extract
7.5 % SDS-PAGE
Bid antibody [N1C3-2] (GTX110568) dilution: 1:1000

Anti-Bid antibody [N1C3-2] used in Western Blot (WB). GTX110568

GTX110568 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:500.

REFERENCE

Application Reference

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Ovadje P et al. Oncotarget 2016; Dandelion root extract affects colorectal cancer proliferation and survival through the activation of multiple death signalling pathways.
Application : WB
Reactivity : Human
Carpentieri A et al. Cell Death Dis 2015; 6 : e1974 Differentiation of human neuroblastoma cells toward the osteogenic lineage by mTOR inhibitor.
Pu YF et al. Int J Clin Exp Pathol 2014; 7 (4) : 1563-71 Generation of homologous cell pairs using the oral lymphatic system.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169