ICC/IF analysis of A549 cells using GTX25498 Bid (p15-specific ) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature

WB analysis of Jurkat cells without added BID (Lanes 1 and 2), with caspase-8 cleaved recombinant human BID (Lane 3), or with caspase-8 cleaved recombinant mouse BID (Lane 4) using either a human BID full length antibody (lane 1) or GTX25498 Bid (p15-specific ) antibody (lanes 2-4). The data show that the human BID (p15) antibody recognizes only the human 15 kDa BID fragment, demonstrating the specificity of the antibody.