Sample(30 μg of whole cell lysate)
A:H1299
7.5% SDS PAGE
GTX104636 diluted at 1:1000

Bif-1 antibody detects Bif-1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Bif-1 antibody (GTX104636) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Bif-1 antibody [C2C3], C-term detects Bif-1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: Bif-1 protein stained by Bif-1 antibody [C2C3], C-term (GTX104636) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX104636 diluted at 1:1000