WB analysis of various samples using GTX48809 Borrelia burgdorferi Dbp-A antibody.
Lane 1 : Protein ladder
Lane 2 : MBP-Dbp-A fusion protein
Lane 3 : Dbp-A protein
Lane 4 : MBP
Loading : 0.1 μg
Dilution : 1:1000

Western blot showing detection of 0.1 μg recombinant proteins in western blot. Lane 1: Molecular weight markers. Lane 2: MBP-DbpA fusion protein (arrow; expected MW: 60.9 kDa). Lane 3: DbpA, MBP removed by TEV cleavage. Lane 4: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4ºC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).