Recommended Starting Dilutions:
>1:1,000 in Western Blot
>1:40,000 for ICC/IF using Avidin-Biotin amplification
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
This antibody reacts with Borrelia burgdorferi (Lyme) (Reactive Western Blot bands at 83kD, 41kD, 34kD and 31kD + additional low MW bands)
Phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. No stabilizing proteins have been added.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
4-5 mg/ml (Please refer to the vial label for the specific concentration.)
Whole cell preparation from B. burgdorferi
Purified by protein A chromatography or sequential differential precipitations
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.