Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Brevican antibody [GT123] (GTX642324) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.