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Brn2 antibody

Anti-Brn2 antibody used in Immunoprecipitation (IP). GTX114650
Anti-Brn2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX114650
Anti-Brn2 antibody used in ChIP assay (ChIP assay). GTX114650
Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650
Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650
Anti-Brn2 antibody used in Western Blot (WB). GTX114650
Anti-Brn2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114650
Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650

Cat No. GTX114650

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay, IHC

Reactivity

Human, Mouse, Rat, Chimpanzee
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

A375

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Brn2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619683

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

POU class 3 homeobox 2 , BRN2 , N-Oct3 , OCT7 , OTF-7 , OTF7 , POUF3 , brn-2 , oct-7

Cellular Localization

Nucleus

Background

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Brn2 antibody used in Immunoprecipitation (IP). GTX114650

GTX114650 IP Image

Brn2 antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 μg of Brn2 antibody (GTX114650) 10% SDS-PAGE The immunoprecipitated Brn2 protein was detected by Brn2 antibody (GTX114650) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Brn2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX114650

GTX114650 IHC-Fr Image

Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Brn2 antibody used in ChIP assay (ChIP assay). GTX114650

GTX114650 ChIP assay Image

Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Brn2 antibody (GTX114650) The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.

Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650

GTX114650 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2(GTX114650) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650

GTX114650 IHC-P Image

Brn2 antibody detects Brn2 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Brn2 antibody (GTX114650) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Brn2 antibody used in Western Blot (WB). GTX114650

GTX114650 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Brn2 antibody (GTX114650) diluted at 1:1000.

Anti-Brn2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114650

GTX114650 ICC/IF Image

Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.

Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650

GTX114650 IHC-P Image

Brn2 antibody detects Brn2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Brn2 antibody (GTX114650) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Brn2 antibody Cat No. GTX114650

Rating ( Average 4.5 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 21-Apr-2019
Score :
Application Tested : ICC/IF
Sample Species : Chmp
Sample : Neural stem cells derived from iPS cells
Blocking : 5% ImmunoBlock (KAC Co.), 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:200, 4°C, 16Hr
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : iPS cell line 201B7-derived neural tissue
Blocking : 2% skim milk in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.3% TritonX in PBS
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169