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Brn2 antibody

Anti-Brn2 antibody used in Immunoprecipitation (IP). GTX114650
Anti-Brn2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX114650
Anti-Brn2 antibody used in ChIP assay (ChIP assay). GTX114650
Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650
Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650
Anti-Brn2 antibody used in Western Blot (WB). GTX114650
Anti-Brn2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114650
Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650

Cat No. GTX114650

Reference 8
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay, IHC

Reactivity

 Human, Mouse, Rat, Chimpanzee
Package
100 μl ($329),
25 μl ($169)
See all Brn2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

A375

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Brn2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

POU class 3 homeobox 2 , BRN2 , N-Oct3 , OCT7 , OTF-7 , OTF7 , POUF3 , brn-2 , oct-7

Cellular Localization

Nucleus

Background

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Brn2 antibody used in Immunoprecipitation (IP). GTX114650

GTX114650 IP Image

Brn2 antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 μg of Brn2 antibody (GTX114650) 10% SDS-PAGE The immunoprecipitated Brn2 protein was detected by Brn2 antibody (GTX114650) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Brn2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX114650

GTX114650 IHC-Fr Image

Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Brn2 antibody used in ChIP assay (ChIP assay). GTX114650

GTX114650 ChIP assay Image

Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Brn2 antibody (GTX114650) The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.

Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650

GTX114650 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2(GTX114650) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650

GTX114650 IHC-P Image

Brn2 antibody detects Brn2 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Brn2 antibody (GTX114650) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Brn2 antibody used in Western Blot (WB). GTX114650

GTX114650 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Brn2 antibody (GTX114650) diluted at 1:1000.

Anti-Brn2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114650

GTX114650 ICC/IF Image

Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.

Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114650

GTX114650 IHC-P Image

Brn2 antibody detects Brn2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Brn2 antibody (GTX114650) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yoon SJ et al. Nat Methods 2019; 16 (1) : 75-78 Reliability of human cortical organoid generation.
Application : IHC-Fr
Reactivity : Human
Enriquez-Rios V et al. J Neurosci 2017; 37 (4) : 893-905 DNA-PKcs, ATM, and ATR Interplay Maintains Genome Integrity during Neurogenesis.
Wong WT et al. Elife 2017; 6 Discovery of novel determinants of endothelial lineage using chimeric heterokaryons.
Application : WB, ChIP assay
Reactivity : Human
Enriquez-Rios V et al. JNeurosci 2016; (Epub) DNA-PKcs, ATM and ATR interplay maintains genome integrity during neurogenesis.
Fujino K et al. Am J Pathol 2015; 185 (12) : 3164-77 *oma-associated protein 1 is a crucial regulator of neuroendocrine differentiation in lung cancer.
Application : WB
Reactivity : Human
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REVIEW

Brn2 antibody Cat No. GTX114650

Rating
( Average 4.5 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4.5 based on 2 users reviews)
Anti-Brn2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114650
Date : Anonymous submitted on 21-Apr-2019
Score :
Application Tested : ICC/IF
Sample Species : Chmp
Sample : Neural stem cells derived from iPS cells
Blocking : 5% ImmunoBlock (KAC Co.), 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:200, 4°C, 16Hr
Anti-Brn2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114650
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : iPS cell line 201B7-derived neural tissue
Blocking : 2% skim milk in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.3% TritonX in PBS
Primary Antibody : 1:500, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169