GeneTex
United States (US)

Brn2 antibody

GTX114650_40212_IP_18121410_730.jpg
GTX114650_40212_20160921_IHC-Fr_18121410_472.jpg
GTX114650_40212_ChIP_18121410_798.jpg
GTX114650_40212_IHC_18121410_530.jpg
GTX114650_42732_20170426_IHC-P_R_18121410_319.jpg
GTX114650_42732_20170412_IFA_18121410_887.jpg
GTX114650_42732_20170426_IHC-P_M_18121410_542.jpg
GTX114650_42487_20160519_WB_18121410_740.jpg

Cat No. GTX114650

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

A375

Predict Reactivity

Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Brn2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pou Class 3 Homeobox 2,Brn2,N-Oct3,Oct7,Otf-7,Otf7,Pouf3,Brn-2,Oct-7,Pou3F2

Cellular Localization

Nucleus

Background

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX114650_40212_IP_18121410_730.jpg

GTX114650 IP Image

Brn2 antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 μg of Brn2 antibody (GTX114650) 10% SDS-PAGE The immunoprecipitated Brn2 protein was detected by Brn2 antibody (GTX114650) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX114650_40212_20160921_IHC-Fr_18121410_472.jpg

GTX114650 IHC-Fr Image

Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX114650_40212_ChIP_18121410_798.jpg

GTX114650 ChIP assay Image

Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Brn2 antibody (GTX114650) The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.

GTX114650_40212_IHC_18121410_530.jpg

GTX114650 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2(GTX114650) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114650_42732_20170426_IHC-P_R_18121410_319.jpg

GTX114650 IHC-P Image

Brn2 antibody detects Brn2 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Brn2 antibody (GTX114650) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114650_42732_20170412_IFA_18121410_887.jpg

GTX114650 ICC/IF Image

Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.

GTX114650_42732_20170426_IHC-P_M_18121410_542.jpg

GTX114650 IHC-P Image

Brn2 antibody detects Brn2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Brn2 antibody (GTX114650) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114650_42487_20160519_WB_18121410_740.jpg

GTX114650 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Brn2 antibody (GTX114650) diluted at 1:1000.

REFERENCE
REVIEW

Brn2 antibody Cat No. GTX114650

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : iPS cell line 201B7-derived neural tissue
Blocking : 2% skim milk in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.3% TritonX in PBS
Primary Antibody : 1:500, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169