Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cross-linked ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or anti-C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter.

C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (GTX100674) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (GTX100674) diluted at 1:500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

The data was published in the journal PLoS One in 2015. PMID: 26121644