Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C5L2 antibody [HL4135] (GTX642940) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled T-47D whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C5L2 antibody [HL4135] (GTX642940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)