GeneTex
United States (US)

C5a (His-Tag) recombinant protein

Cat No. GTX65515

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Human recombinant C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase. The current lot of ED50 = 1.36 nmolar.

Calculated MW

(74 amino acids) kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute to a concentration of 1.0 mg/ml in ice-cold water containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70ºC for future use. Frozen/thaw will result in some loss of activity.

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Human complement C5a is a 74-amino acid glycopeptide generated during complement activation from the a-chain of complement C5. C5a is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. The 74 amino acid, human C5a protein expressed in E. coli with a 6 amino acid, N-terminal His·Tag sequence and 28 amino acid proprietary vector sequence necessary for expression (C5a without His-tag is alailable, GeneTex Cat # GTX65516). The recombinant protein is not further processed to remove the additional sequence. Recombinant C5a has an activity similar to that of serum derived C5a based on enzyme assay of myeloperoxidase release.
Package List Price ($)
$ 259