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CCK4 antibody [N1N2], N-term

Anti-CCK4 antibody [N1N2], N-term used in Western Blot (WB). GTX113816
Anti-CCK4 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113816
Anti-CCK4 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113816

Cat No. GTX113816

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CCK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721592

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein tyrosine kinase 7 (inactive) , CCK-4 , CCK4

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CCK4 antibody [N1N2], N-term used in Western Blot (WB). GTX113816

GTX113816 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCK4 antibody [N1N2], N-term (GTX113816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCK4 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113816

GTX113816 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CCK4(GTX113816) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CCK4 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113816

GTX113816 ICC/IF Image

CCK4 antibody [N1N2], N-term detects CCK4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CCK4 protein stained by CCK4 antibody [N1N2], N-term (GTX113816) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

There are currently no references for CCK4 antibody [N1N2], N-term (GTX113816). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CCK4 antibody [N1N2], N-term (GTX113816). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169