APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.1-0.2 μg/mL |
0.5-1.0 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
The antibody has the ability to neutralize the biological activity of recombinant human 6Ckine. It exhibits less than 15% cross-reactivity with recombinant mouse 6Ckine and less than 5% cross-reactivity with recombinant human fractalkine, Teck, MCP-3, mouse CRG-2, and human and mouse VIC.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
recombinant human 6Ckine, expressed in Escherichia coli.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-C motif chemokine ligand 21 , 6Ckine , CKb9 , ECL , SCYA21 , SLC , TCA4
Cellular Localization
Secreted
Background
Human 6Ckine is a beta or C-C chemokine identified in the Expressed Sequence Tag (EST) database by three independent groups. Known also as Exodus2 and secondary lymphoid-tissue chemokine (SLC), 6Ckine contains four conserved cysteine (C) residues which are characteristic of beta-chemokines. Two additional conserved cysteine residues have been found in its unusually long carboxy-terminal domain and consequently the name 6Ckine. Human and mouse 6Ckine are highly conserved and show 86% amino acid homology. Human 6Ckine cDNA encodes a 134 amino acid precursor protein, a 23 amino acid signal peptide and a 111 amino acid mature protein. This protein shares 21 % to 33 % homology with other human C-C chemokines. Comparatively, mouse 6Ckine cDNA encodes a 133 amino acid precursor protein, a 23 amino acid signal peptide and a 110 amino acid mature protein. Recombinant human 6Ckine has a predicted molecular mass of approximately 12 kDa (mature protein). In SDS-PAGE under reducing and non-reducing conditions, the recombinant protein migrates with an apparent molecular mass of 16 kDa to 17 kDa. The human 6Ckine gene has been mapped to chromosome 9p13. The expression of human 6Ckine has been detected primarily in lymphoid tissues but also in the gastrointestinal tract. Recombinant human 6Ckine is chemotactic for some human T-cell lines, resting peripheral blood lymphocytes, and normal cultured T-cells treated with PHA and IL2. Unlike other C-C chemokines, 6Ckine is not chemotactic for monocytes and neutrophils. A growing body of work suggests that 6Ckine influences lymphocyte homing to secondary lymphoid organs, integrin-mediated lymphocyte adhesion, and may act via the EBI1 ligand chemokine (ELC) receptor, CCR7.
Database
Research Area
REFERENCE
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REVIEW
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