CCR6 antibody [53103.111]

Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The a- and b- families, with four cysteine residues, are the largest and best characterized. In the a-family, one amino acid separates the first two cysteine residues (CXC); in the b-family the two cysteine residues (CC) are adjacent to each other. The a-chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL-8), while those that do not, act on lymphocytes (such as IP-10 and MIG). Examples of chemokines under the b-family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the g-family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the *-family and has the first two cysteine residues separated by three amino-acids (CXXXC).Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells.1 Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain.Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient.CCR-6 (previously referred to as the GPR-CY4, CKRL3, or STRL22 orphan receptor) binds MIP-3". It is a CC chemokine receptor that is highly expressed in human dendritic cells derived from CD34+ cord blood precursors.2-4 In addition to dendritic cells, CCR-6 mRNA has also been detected in lymphocytes (CD4 and CD8 T cells and B cells), but not in natural killer cells, monocytes and granulocytes.