Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR7 antibody [HL3075] (GTX640521) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CCR7 antibody [HL3075] detects CCR7 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
CCR7 stained by CCR7 antibody [HL3075] (GTX640521) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min