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CD13 antibody

Overexpression
Anti-CD13 antibody used in Western Blot (WB). GTX102788
Anti-CD13 antibody used in Western Blot (WB). GTX102788
Anti-CD13 antibody used in IHC (Paraffin sections) (IHC-P). GTX102788
Anti-CD13 antibody used in Western Blot (WB). GTX102788

Cat. No. GTX102788

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

THP-1 , HL-60 , ANPEP-transfected 293T

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949656

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alanyl aminopeptidase, membrane , APN , CD13 , GP150 , LAP1 , P150 , PEPN

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Cytoplasm , cytosol , Cell membrane , Cytoplasm , cytosol

Background

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD13 antibody used in Western Blot (WB). GTX102788

GTX102788 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CD13 antibody (GTX102788) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CD13 antibody used in Western Blot (WB). GTX102788

GTX102788 WB Image

CD13 antibody detects CD13 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and blotted with CD13 antibody (GTX102788) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD13 antibody used in IHC (Paraffin sections) (IHC-P). GTX102788

GTX102788 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using CD13(GTX102788) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD13 antibody used in Western Blot (WB). GTX102788

GTX102788 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD13 antibody (GTX102788) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kovak MR et al. Am J Reprod Immunol 2014; 72 (4) : 403-12 Investigation of galectin-3 function in the reproductive tract by identification of binding ligands in human seminal plasma.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169