GeneTex
United States (US)

CD133 antibody [C1C2], Internal

Overexpression
GTX100567_39471_WB_B_19011506_797.jpg
GTX100567_39471_IHC_19011506_445.jpg
GTX100567_39471_WB_19011506_849.jpg

Cat No. GTX100567

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

HCT116 , human CD133-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AC133 Antibody, MSTP061 Antibody, PROM1 Antibody, CORD12 Antibody, RP41 Antibody, STGD4 Antibody, MCDR2 Antibody, PROML1 Antibody

Cellular Localization

Apical cell membrane; Multi-pass membrane protein (By similarity) , Cell projection , microvillus membrane; Multi-pass membrane protein

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100567_39471_WB_B_19011506_797.jpg

GTX100567 WB Image

Overexpression

CD133 antibody [C1C2], Internal detects CD133 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human CD133-transfected 293T cells
7.5 % SDS-PAGE
CD133 antibody [C1C2], Internal (GTX100567) dilution: 1:5000

GTX100567_39471_IHC_19011506_445.jpg

GTX100567 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using CD133(GTX100567) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100567_39471_WB_19011506_849.jpg

GTX100567 WB Image

CD133 antibody detects CD133 protein by western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract
7.5 % SDS-PAGE
CD133 antibody (GTX100567) dilution: 1:500

REFERENCE
REVIEW

CD133 antibody [C1C2], Internal Cat No. GTX100567

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, overnightHr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysate
Date : Anonymous submitted on 07-Jan-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : A549 cells
Primary Antibody : 1:200, 4°C, 0.25Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169