CD133 antibody [C1C2], Internal

Cat No. GTX100567

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, FACS
Reactivity	Human

See all CD133 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:10000
IHC-P	1:100-1:1000
FACS	Assay dependent

Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

HCT116 , human CD133-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Prominin 1,Ac133,Cd133,Cord12,Mcdr2,Mstp061,Proml1,Rp41,Stgd4,Prom1

Cellular Localization

Apical cell membrane; Multi-pass membrane protein , Cell projection , microvillus membrane; Multi-pass membrane protein

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 8842
UniProt: O43490

Research Area

DATA IMAGES

GTX100567 WB Image

CD133 antibody [C1C2], Internal detects CD133 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human CD133-transfected 293T cells
7.5 % SDS-PAGE
CD133 antibody [C1C2], Internal (GTX100567) dilution: 1:5000

GTX100567 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using CD133(GTX100567) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100567 WB Image

CD133 antibody detects CD133 protein by western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract
7.5 % SDS-PAGE
CD133 antibody (GTX100567) dilution: 1:500

REFERENCE

Application Reference

Ma L et al. PLoS One 2017; 12 (11) : e0188665 Suppression of cancer stemness by upregulating Ligand-of-Numb protein X1 in colorectal carcinoma.

Application : WB

Reactivity : Human

Huang WT et al. Eur J Pharm Biopharm 2016; 109 : 165-173 Dual Drug-Loaded Biofunctionalized Amphiphilic Chitosan Nanoparticles: Enhanced Synergy between Cisplatin and Demethoxycurcumin against Multidrug-Resistant Stem-Like Lung Cancer Cells.

Huang WT et al. Eur J Pharm Biopharm 2016; (Epub ahead of print) Dual Drug-Loaded Biofunctionalized Amphiphilic Chitosan Nanoparticles: Enhanced Synergy between Cisplatin and Demethoxycurcumin against Multidrug-Resistant Stem-Like Lung Cancer Cells.

Qiu HO et al. Med Sci Monit 2016; 22 : 1146-51 Identification and Characterization of CD133pos Subpopulation Cells From a Human Laryngeal Cancer Cell Line.

Application : FACS

Reactivity : Human

Submit a Reference

REVIEW

CD133 antibody [C1C2], Internal Cat No. GTX100567

Rating

( Average 5 based on 2 users reviews)

Application

Western Blot(WB)		( Average 5 based on 1 users reviews)
Flow cytometry(FACS)		( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 26-May-2018

Score :

Application Tested : WB

Sample Species : Hu

Sample : MG63 cells

Amount used : 30 μg

Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr

Primary Antibody : 1:100, 4°C, overnightHr

Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysate