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CD133 antibody [C1C2], Internal

Overexpression
Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567
Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567
Anti-CD133 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100567

Cat No. GTX100567

Reference 4
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all CD133 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

NT2D1

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.00 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD133. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

prominin 1 , AC133 , CD133 , CORD12 , MCDR2 , MSTP061 , PROML1 , RP41 , STGD4

Cellular Localization

Apical cell membrane,Endoplasmic reticulum

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567

GTX100567 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [C1C2], Internal (GTX100567) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 29760280

Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567

GTX100567 WB Image

Overexpression

CD133 antibody [C1C2], Internal detects CD133 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human CD133-transfected 293T cells
7.5 % SDS-PAGE
CD133 antibody [C1C2], Internal (GTX100567) dilution: 1:5000

Anti-CD133 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100567

GTX100567 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using CD133(GTX100567) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Wu HY et al. Cancer Lett 2019; 456 : 13-22 p21-Activated kinase 3 promotes cancer stem cell phenotypes through activating the Akt-GSK3β-β-catenin signaling pathway in pancreatic cancer cells.
Application : WB
Reactivity : Human
Ma L et al. PLoS One 2017; 12 (11) : e0188665 Suppression of cancer stemness by upregulating Ligand-of-Numb protein X1 in colorectal carcinoma.
Application : WB
Reactivity : Human
Huang WT et al. Eur J Pharm Biopharm 2016; (Epub) dual drug-loaded biofunctionalized amphiphilic chitosan nanoparticles: enhanced synergy between * and demethoxycurcumin against multidrug-resistant stem-like lung cancer cells.
Qiu HO et al. Med Sci Monit 2016; 22 : 1146-51 Identification and Characterization of CD133pos Subpopulation Cells From a Human Laryngeal Cancer Cell Line.
Application : FACS
Reactivity : Human
Submit a Reference

REVIEW

CD133 antibody [C1C2], Internal Cat No. GTX100567

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Anti-CD133 antibody [C1C2], Internal used in Western Blot (WB). GTX100567
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysate
Anti-CD133 antibody [C1C2], Internal used in Flow cytometry (FACS). GTX100567
Date : Anonymous submitted on 07-Jan-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : A549 cells
Primary Antibody : 1:200, 4°C, 0.25Hr
Submit a Review
Package List Price ($)
$ 329
$ 169