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CD133 antibody

Anti-CD133 antibody used in IHC (Paraffin sections) (IHC-P). GTX12295
Anti-CD133 antibody used in Western Blot (WB). GTX12295
Anti-CD133 antibody used in Western Blot (WB). GTX12295

Cat No. GTX12295

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μg ($289)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

97.2 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

2.5% BSA, 0.45% NaCl, 0.1% Na₂HPO₄, 0.025% Thimerosal, 0.025% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PROM1(836-850aa MKNMENGNNGYHKDH), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Apical cell membrane,Cell projection,Microvillus membrane,Cell projection,Cilium,Photoreceptor outer segment,Endoplasmic reticulum,Endoplasmic reticulum-Golgi intermediate compartment,Apical cell membrane,Cell projection,Cell projection (by similarity),Cilium (by similarity),Microvillus membrane,Photoreceptor outer segment (by similarity),Apical cell membrane,Cell projection,Cell projection (by similarity),Cilium (by similarity),Microvillus membrane,Photoreceptor outer segment (by similarity),Apical cell membrane,Cell projection,Cell projection (by similarity),Cilium (by similarity),Microvillus membrane,Photoreceptor outer segment (by similarity),Apical cell membrane,Cell projection,Cell projection (by similarity),Cilium (by similarity),Microvillus membrane,Photoreceptor outer segment (by similarity),Apical cell membrane,Cell projection,Cell projection (by similarity),Cilium (by similarity),Microvillus membrane,Photoreceptor outer segment (by similarity)

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES
Anti-CD133 antibody used in IHC (Paraffin sections) (IHC-P). GTX12295

GTX12295 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX12295 CD133 antibody.

Anti-CD133 antibody used in Western Blot (WB). GTX12295

GTX12295 WB Image

WB analysis of various samples using GTX12295 CD133 antibody.
Lane 1 : PANC cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SW620 cell lysate
Lane 4 : COLO320 cell lysate
Lane 5 : A549 cell lysate

Anti-CD133 antibody used in Western Blot (WB). GTX12295

GTX12295 WB Image

WB analysis of various samples using GTX12295 CD133 antibody.
Lane 1 : rat liver tissue lysates
Lane 2 : mouse liver tissue lysates
Lane 3 : mouse lung tissue lysates
Lane 4 : rat brain tissue lysates
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

REFERENCE
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Package List Price ($)
$ 289