GeneTex
United States (US)

CD133 antibody

Anti-CD133 antibody used in Western Blot (WB). GTX12295
Anti-CD133 antibody used in Western Blot (WB). GTX12295
Anti-CD133 antibody used in IHC (Paraffin sections) (IHC-P). GTX12295

Cat No. GTX12295

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

IHC-P: 0.5-1ug/ml; WB: 0.1-0.5ug/ml

Calculated MW

97 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

5% BSA, 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% NaN3.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 836 and 850 of human CD133.

Purification

Immunogen affinity purified.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Prominin 1,Ac133,Cd133,Cord12,Mcdr2,Mstp061,Proml1,Rp41,Stgd4,Prom1

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES
Anti-CD133 antibody used in Western Blot (WB). GTX12295

GTX12295 WB Image

Western blot analysis of PROM1 using anti- PROM1 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PROM1 antigen affinity purified polyclonal antibody at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection with Tanon 5200 system. A specific band was detected for PROM1 at approximately 130KD. The expected band size for PROM1 is at 97KD.

Anti-CD133 antibody used in Western Blot (WB). GTX12295

GTX12295 WB Image

Lane 1: 22KV1 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: 293T Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 5: MCF-7 Cell Lysate

Anti-CD133 antibody used in IHC (Paraffin sections) (IHC-P). GTX12295

GTX12295 IHC-P Image

IHC-P of human tonsil tissue

Package List Price ($)
$ 289