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CD133 antibody

Anti-CD133 antibody used in Immunoprecipitation (IP). GTX64328
Anti-CD133 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64328
Anti-CD133 antibody used in Western Blot (WB). GTX64328
Anti-CD133 antibody used in Western Blot (WB). GTX64328
Anti-CD133 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64328

Cat No. GTX64328

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:3000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 750-850 of human PROM1 (NP_006008.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

prominin 1 , AC133 , CD133 , CORD12 , MCDR2 , MSTP061 , PROML1 , RP41 , STGD4

Cellular Localization

Apical cell membrane,Endoplasmic reticulum

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD133 antibody used in Immunoprecipitation (IP). GTX64328

GTX64328 IP Image

IP analysis of HT-29 cell lysate using GTX64328 CD133 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-CD133 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64328

GTX64328 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64328 CD133 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD133 antibody used in Western Blot (WB). GTX64328

GTX64328 WB Image

WB analysis of various sample lysates using GTX64328 CD133 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-CD133 antibody used in Western Blot (WB). GTX64328

GTX64328 WB Image

WB analysis of normal (control) and knockout (KO) HCT116 cell lysate using GTX64328 CD133 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD133 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64328

GTX64328 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX64328 CD133 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339