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CD166 antibody [AZN-L50] (Low endotoxin, azide free)

Cat No. GTX18159

Host

Mouse

Clonality

Monoclonal

Clone Name

AZN-L50

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Neutralizing/Inhibition

Reactivity

Human
Package
250 μg ($699)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 4 μg/ml
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 4μg/ml antibody / 2x10⁵ K562 cells
IP Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

WB
A non-reduced sample treatment and 6.5 % SDS-Page was used.

ICC/IF
Antibody AZN-L50 was used on methanol/acetone fixed monolayers of cells grown on a glass surface.

IHC-P
Tissue sections were pretreated by heating in a rice steamer in citrate buffer for 10 minutes for antigen retrieval. Tissue sections were blocked with normal horse serum.

IHC-Fr
Tissue sections were fixed in acetone

IP
Radioactive labelled KG1a cells were lysed and precipitated with 1 μg of antibody coupled to Protein G Sepharose 4 Fast Flow beads.

Neutralizing/Inhibition
Antibody AZN-L50 functions as an blocking antibody for homotypic ALCAM-ALCAM interaction.

Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Product Note

This antibody stains the extracellular domain of ALCAM. The monoclonal antibody AZN-L50 completely blocks homophilic ALCAM-ALCAM interaction.

PROPERTIES

Form

Liquid

Buffer

0.2μm-filtered PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CD166 expressing K562 cells

Purification

Protein G purified

Endotoxin

< 24 EU/mg (determined with HIT302 LAL Assay)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ALCAM,CD166,MEMD,activated leukocyte cell adhesion molecule

Cellular Localization

Cell membrane,Cell projection,Axon,Dendrite,Secreted

Background

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 699
Custom Conjugations