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CD19 antibody [HIB19] (PE-Cy7)

Anti-CD19 antibody [HIB19] (PE-Cy7) used in Flow cytometry (FACS). GTX01455-10

Cat. No. GTX01455-10

Reference 1684

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HIB19

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
100 test ($259)
See all CD19 products
PRODUCT

Summary

The HIB19 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21, CD81 and CD225, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.The HIB19 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄ (pH7.2), 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin-Cyanine7 (PE-Cy7)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD19 molecule , B4 , CD19 , CVID3

Cellular Localization

Membrane

Background

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD19 antibody [HIB19] (PE-Cy7) used in Flow cytometry (FACS). GTX01455-10

GTX01455-10 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01455-10 CD19 antibody [HIB19] (PE-Cy7).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)

REFERENCE

Clone Reference

Benjelloun F et al. Front Immunol 2020; 11:1655 Activation of Toll-Like Receptors Differentially Modulates Inflammation in the Human Reproductive Tract: Preliminary Findings.
Bourdely P et al. Immunity 2020; 53 (2):335-352.e8 Transcriptional and Functional Analysis of CD1c+ Human Dendritic Cells Identifies a CD163+ Subset Priming CD8+CD103+ T Cells.
Salzer B et al. Nat Commun 2020; 11 (1):4166 Engineering AvidCARs for combinatorial antigen recognition and reversible control of CAR function.
Imura Y et al. JCI Insight 2020; 5 (14) CD19-targeted CAR regulatory T cells suppress B cell pathology without GvHD.
Zhao NQ et al. PLoS One 2020; 15 (9):e0238347 Natural killer cell phenotype is altered in HIV-exposed seronegative women.
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Clone Reference
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