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CD272 antibody

Anti-CD272 antibody used in Western Blot (WB). GTX112783
Anti-CD272 antibody used in IHC (Paraffin sections) (IHC-P). GTX112783

Cat No. GTX112783

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

A375
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD272. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B and T lymphocyte associated,BTLA1,CD272

Cellular Localization

Membrane

Background

BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 (MIM 600244) and CTLA4 (MIM 123890), BTLA interacts with a B7 homolog, B7H4.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-CD272 antibody used in Western Blot (WB). GTX112783

GTX112783 WB Image

CD272 antibody detects BTLA protein by Western blot analysis.
A. 30 µg A375 whole cell lysate/extract
10 % SDS-PAGE
CD272 antibody (GTX112783) dilution: 1:1000

Anti-CD272 antibody used in IHC (Paraffin sections) (IHC-P). GTX112783

GTX112783 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using CD272(GTX112783) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf